CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020843
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 1,4-alpha-glucan-branching enzyme 
Protein Synonyms/Alias
 Brancher enzyme; Glycogen-branching enzyme 
Gene Name
 Gbe1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
39LEIDPYLKPFAADFQubiquitination[1]
51DFQRRYKKFSQVLHDubiquitination[1]
68ENEGGIDKFSRGYESubiquitination[1]
121YKKLEYGKWELYIPPubiquitination[1]
143IPHGSKLKVVITSKSubiquitination[1]
149LKVVITSKSGEILYRubiquitination[1]
162YRISPWAKYVVRENNubiquitination[1]
212GISSHEGKIASYKHFubiquitination[1]
442LAMAIPDKWIQLLKEubiquitination[1]
472TNRRYLEKCVAYAESubiquitination[1]
578DDDLLRYKFLNNFDRubiquitination[1]
645VGTATPGKFKIVLDSacetylation[2]
645VGTATPGKFKIVLDSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758] 
Functional Description
 Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells (By similarity). 
Sequence Annotation
 ACT_SITE 357 357 Nucleophile (By similarity).
 ACT_SITE 412 412 Proton donor (By similarity).
 MOD_RES 173 173 Phosphotyrosine (By similarity).  
Keyword
 Complete proteome; Glycogen biosynthesis; Glycogen storage disease; Glycosyltransferase; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 702 AA 
Protein Sequence
MAAPAAPAAG ETGPDARLEA ALADVPELAR LLEIDPYLKP FAADFQRRYK KFSQVLHDIG 60
ENEGGIDKFS RGYESFGIHR CSDGGIYCKE WAPGAEGVFL TGEFSGWNPF SHPYKKLEYG 120
KWELYIPPKQ NKSPLIPHGS KLKVVITSKS GEILYRISPW AKYVVRENNN VNYDWIHWAP 180
EDPYKFKHSR PKKPRSLRIY ESHVGISSHE GKIASYKHFT SNVLPRIKDL GYNCIQLMAI 240
MEHAYYASFG YQITSFFAAS SRYGTPEELK ELVDTAHSMG IVVLLDVVHS HASKNSEDGL 300
NMFDGTDSCY FHSGPRGTHD LWDSRLFIYS SWEVLRFLLS NIRWWLEEYC FDGFRFDGVT 360
SMLYHHHGMG QGFSGDYNEY FGLQVDEDAL IYLMLANHLA HTLYPDSITI AEDVSGMPAL 420
CSPTSQGGGG FDYRLAMAIP DKWIQLLKEF KDEDWNMGNI VYTLTNRRYL EKCVAYAESH 480
DQALVGDKTL AFWLMDAEMY TNMSVLAPFT PVIDRGIQLH KMIRLITHGL GGEGYLNFMG 540
NEFGHPEWLD FPRKGNNESY HYARRQFNLT DDDLLRYKFL NNFDRDMNRL EERCGWLSAP 600
QAYVSEKHEA NKTITFERAG LLFIFNFHPS KSYTDYRVGT ATPGKFKIVL DSDAAEYGGH 660
QRLDHNTNYF AEAFEHNGRP YSLLVYIPSR VALILQNVDL QN 702 
Gene Ontology
 GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:EC.
 GO:0043169; F:cation binding; IEA:InterPro.
 GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
 GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR006407; 1-4-A-glucan_branch_enz.
 IPR006048; A-amylase_b_C.
 IPR015902; Glyco_hydro_13.
 IPR013780; Glyco_hydro_13_b.
 IPR006047; Glyco_hydro_13_cat_dom.
 IPR004193; Glyco_hydro_13_N.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF.
 IPR013783; Ig-like_fold.
 IPR014756; Ig_E-set. 
Pfam
 PF00128; Alpha-amylase
 PF02806; Alpha-amylase_C
 PF02922; CBM_48 
SMART
  
PROSITE
  
PRINTS