UniProt Accession

 TSAA_ECOLI; P28634; Q47676 

Genbank Protein ID

 D15061; L04474; U70214; U00096; AP009048 

Genbank Nucleotide ID

 BAA03655.1; AAA24509.1; AAB08623.1; AAC73306.1; BAA77871.1 

Protein Name

 Probable tRNA (adenine(37)-N6)-methyltransferase 

Protein Synonyms/Alias

 tRNA (m6t6A37) methyltransferase 

Gene Name

 tsaA 

Gene Synonyms/Alias

 yaeB; b0195; JW0191 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
114	LKEVVCHKDSVILKL	acetylation	[1]
178	KQLLTLEKRYPQLTL	acetylation	[1]
207	RKGEETGKTYAVWLH	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Is likely the methyltransferase responsible for the addition of the methyl group in the formation of N6-methyl-N6- threonylcarbamoyladenosine at position 37 (m6t6A37) in some tRNAs species that read codons starting with adenosine. The methyl group of m6t6A37 appears to slightly improve the efficiency of the tRNA. 

Sequence Annotation

 DOMAIN 6 147 TsaA-like.
 REGION 23 25 S-adenosyl-L-methionine binding (By
 REGION 64 65 S-adenosyl-L-methionine binding (By
 REGION 127 130 S-adenosyl-L-methionine binding (By
 BINDING 92 92 S-adenosyl-L-methionine (By similarity).  

Keyword

 Complete proteome; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 235 AA 

Protein Sequence

Gene Ontology

 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. 

Interpro

 IPR001378; UPF0066.
 IPR023368; UPF0066_cons_site.
 IPR023370; UPF0066_YaeB. 

Pfam

 PF01980; UPF0066 

SMART

  

PROSITE

 PS01318; TSAA_1
 PS51668; TSAA_2 

PRINTS