CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020073
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SH2 domain-containing protein 3A 
Protein Synonyms/Alias
 Novel SH2-containing protein 1 
Gene Name
 SH2D3A 
Gene Synonyms/Alias
 NSP1; UNQ175/PRO201 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24HGLLSRQKAEALLQQubiquitination[1]
451LAFEQELKPLMRALDubiquitination[1, 2]
560PRAERFEKFQRVLGVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 May play a role in JNK activation. 
Sequence Annotation
 DOMAIN 15 114 SH2.
 MOD_RES 123 123 Phosphoserine.
 MOD_RES 125 125 Phosphoserine.
 MOD_RES 147 147 Phosphoserine.
 MOD_RES 180 180 Phosphoserine.  
Keyword
 Complete proteome; Phosphoprotein; Polymorphism; Reference proteome; SH2 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 576 AA 
Protein Sequence
MQVPQDGEDL AGQPWYHGLL SRQKAEALLQ QNGDFLVRAS GSRGGNPVIS CRWRGSALHF 60
EVFRVALRPR PGRPTALFQL EDEQFPSIPA LVHSYMTGRR PLSQATGAVV SRPVTWQGPL 120
RRSFSEDTLM DGPARIEPLR ARKWSNSQPA DLAHMGRSRE DPAGMEASTM PISALPRTSS 180
DPVLLKAPAP LGTVADSLRA SDGQLQAKAP TKPPRTPSFE LPDASERPPT YCELVPRVPS 240
VQGTSPSQSC PEPEAPWWEA EEDEEEENRC FTRPQAEISF CPHDAPSCLL GPQNRPLEPQ 300
VLHTLRGLFL EHHPGSTALH LLLVDCQATG LLGVTRDQRG NMGVSSGLEL LTLPHGHHLR 360
LELLERHQTL ALAGALAVLG CSGPLEERAA ALRGLVELAL ALRPGAAGDL PGLAAVMGAL 420
LMPQVSRLEH TWRQLRRSHT EAALAFEQEL KPLMRALDEG AGPCDPGEVA LPHVAPMVRL 480
LEGEEVAGPL DESCERLLRT LHGARHMVRD APKFRKVAAQ RLRGFRPNPE LREALTTGFV 540
RRLLWGSRGA GAPRAERFEK FQRVLGVLSQ RLEPDR 576 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0005070; F:SH3/SH2 adaptor activity; TAS:ProtInc.
 GO:0007254; P:JNK cascade; TAS:ProtInc.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR023578; Ras_GEF_dom.
 IPR001895; RasGRF_CDC25.
 IPR000980; SH2. 
Pfam
 PF00617; RasGEF
 PF00017; SH2 
SMART
 SM00252; SH2 
PROSITE
 PS50001; SH2 
PRINTS
 PR00401; SH2DOMAIN.