CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007624
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Excitatory amino acid transporter 1 
Protein Synonyms/Alias
 Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 
Gene Name
 SLC1A3 
Gene Synonyms/Alias
 EAAT1; GLAST; GLAST1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
38KKVQNITKEDVKSYLacetylation[1]
38KKVQNITKEDVKSYLubiquitination[2]
79RMSYREVKYFSFPGEubiquitination[2, 3]
384EENNGVDKRVTRFVLubiquitination[4]
503HLSRHELKNRDVEMGubiquitination[2]
520VIEENEMKKPYQLIAubiquitination[2, 3, 5]
521IEENEMKKPYQLIAQubiquitination[2, 3, 5, 6]
534AQDNETEKPIDSETKubiquitination[2, 3, 5]
541KPIDSETKM******ubiquitination[2, 3, 5]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. 
Sequence Annotation
 MOD_RES 512 512 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Disease mutation; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 542 AA 
Protein Sequence
MTKSNGEEPK MGGRMERFQQ GVRKRTLLAK KKVQNITKED VKSYLFRNAF VLLTVTAVIV 60
GTILGFTLRP YRMSYREVKY FSFPGELLMR MLQMLVLPLI ISSLVTGMAA LDSKASGKMG 120
MRAVVYYMTT TIIAVVIGII IVIIIHPGKG TKENMHREGK IVRVTAADAF LDLIRNMFPP 180
NLVEACFKQF KTNYEKRSFK VPIQANETLV GAVINNVSEA METLTRITEE LVPVPGSVNG 240
VNALGLVVFS MCFGFVIGNM KEQGQALREF FDSLNEAIMR LVAVIMWYAP VGILFLIAGK 300
IVEMEDMGVI GGQLAMYTVT VIVGLLIHAV IVLPLLYFLV TRKNPWVFIG GLLQALITAL 360
GTSSSSATLP ITFKCLEENN GVDKRVTRFV LPVGATINMD GTALYEALAA IFIAQVNNFE 420
LNFGQIITIS ITATAASIGA AGIPQAGLVT MVIVLTSVGL PTDDITLIIA VDWFLDRLRT 480
TTNVLGDSLG AGIVEHLSRH ELKNRDVEMG NSVIEENEMK KPYQLIAQDN ETEKPIDSET 540
KM 542 
Gene Ontology
 GO:0042995; C:cell projection; IEA:Compara.
 GO:0009986; C:cell surface; IEA:Compara.
 GO:0043205; C:fibril; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0016595; F:glutamate binding; IEA:Compara.
 GO:0005314; F:high-affinity glutamate transmembrane transporter activity; IEA:Compara.
 GO:0005313; F:L-glutamate transmembrane transporter activity; TAS:ProtInc.
 GO:0017153; F:sodium:dicarboxylate symporter activity; IEA:InterPro.
 GO:0031223; P:auditory behavior; IEA:Compara.
 GO:0048667; P:cell morphogenesis involved in neuron differentiation; IEA:Compara.
 GO:0021545; P:cranial nerve development; IEA:Compara.
 GO:0070779; P:D-aspartate import; IDA:UniProtKB.
 GO:0009449; P:gamma-aminobutyric acid biosynthetic process; IEA:Compara.
 GO:0006537; P:glutamate biosynthetic process; IEA:Compara.
 GO:0051938; P:L-glutamate import; IDA:UniProtKB.
 GO:0050885; P:neuromuscular process controlling balance; IEA:Compara.
 GO:0001504; P:neurotransmitter uptake; TAS:ProtInc.
 GO:0050806; P:positive regulation of synaptic transmission; IEA:Compara.
 GO:0046677; P:response to antibiotic; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0009416; P:response to light stimulus; IEA:Compara.
 GO:0009611; P:response to wounding; IEA:Compara.
 GO:0007605; P:sensory perception of sound; IEA:Compara. 
Interpro
 IPR001991; Na-dicarboxylate_symporter.
 IPR018107; Na-dicarboxylate_symporter_CS. 
Pfam
 PF00375; SDF 
SMART
  
PROSITE
 PS00713; NA_DICARBOXYL_SYMP_1
 PS00714; NA_DICARBOXYL_SYMP_2 
PRINTS
 PR00173; EDTRNSPORT.