UniProt Accession

 HXKA_YEAST; P04806; D6VTT6 

Genbank Protein ID

 M14410; X03482; D50617; BK006940 

Genbank Nucleotide ID

 AAA34698.1; CAA27202.1; BAA09292.1; DAA12496.1 

Protein Name

 Hexokinase-1 

Protein Synonyms/Alias

 Hexokinase PI; Hexokinase-A 

Gene Name

 HXK1 

Gene Synonyms/Alias

 HKA; YFR053C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
13	PKKPQARKGSMADVP	ubiquitination	[1]
46	ETLRKVVKHFIDELN	acetylation	[2]
54	HFIDELNKGLTKKGG	acetylation	[2]
111	TFDTTQSKYKLPHDM	acetylation	[2]
111	TFDTTQSKYKLPHDM	ubiquitination	[1]
113	DTTQSKYKLPHDMRT	acetylation	[2]
165	SYPASQNKINEGILQ	ubiquitination	[1]
284	HLVLPRTKYDVAVDE	acetylation	[2, 3]
284	HLVLPRTKYDVAVDE	ubiquitination	[1]
367	DTDDIFQKDFGVKTT	ubiquitination	[1, 4]
372	FQKDFGVKTTLPERK	ubiquitination	[1]
423	ADGSVYNKYPGFKEA	acetylation	[2]
423	ADGSVYNKYPGFKEA	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
 Xie H, Bandhakavi S, Roe MR, Griffin TJ.
 J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

  

Sequence Annotation

 NP_BIND 86 91 ATP (By similarity).
 NP_BIND 307 308 ATP (By similarity).
 NP_BIND 344 348 ATP (By similarity).
 NP_BIND 419 423 ATP (By similarity).
 REGION 175 176 Substrate binding.
 REGION 210 211 Substrate binding.
 BINDING 111 111 ATP (Potential).
 BINDING 158 158 Substrate; via carbonyl oxygen.
 BINDING 237 237 Substrate.
 BINDING 269 269 Substrate.
 BINDING 302 302 Substrate.
 MOD_RES 245 245 Phosphoserine.
 MOD_RES 272 272 Phosphoserine.  

Keyword

 3D-structure; Allosteric enzyme; ATP-binding; Complete proteome; Direct protein sequencing; Glycolysis; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 485 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004396; F:hexokinase activity; IDA:SGD.
 GO:0032445; P:fructose import; IGI:SGD.
 GO:0006000; P:fructose metabolic process; IMP:SGD.
 GO:0046323; P:glucose import; IGI:SGD.
 GO:0006096; P:glycolysis; IDA:SGD.
 GO:0006013; P:mannose metabolic process; IDA:SGD. 

Interpro

 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 

Pfam

 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 

SMART

  

PROSITE

 PS00378; HEXOKINASES 

PRINTS

 PR00475; HEXOKINASE.