CPLM-008456

Genbank Nucleotide ID

Ubiquitin-conjugating enzyme E2 E1

Protein Synonyms/Alias

UbcH6; Ubiquitin carrier protein E1; Ubiquitin-protein ligase E1

Homo sapiens (Human)

Position	Peptide	Type	References
24	SSNQQTEKETNTPKK	ubiquitination	[1, 2]
43	VSMSKNSKLLSTSAK	ubiquitination	[1, 3]
50	KLLSTSAKRIQKELA	ubiquitination	[3]
54	TSAKRIQKELADITL	ubiquitination	[4]
136	VICLDILKDNWSPAL	ubiquitination	[1, 2, 5]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[5] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
Xu G, Paige JS, Jaffrey SR.
Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]

Functional Description

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.

ACT_SITE 131 131 Glycyl thioester intermediate (By
MOD_RES 2 2 N-acetylserine.
CROSSLNK 136 136 Glycyl lysine isopeptide (Lys-Gly)

3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Isopeptide bond; Ligase; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; Ubl conjugation; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0000151; C:ubiquitin ligase complex; IDA:UniProtKB.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0042296; F:ISG15 ligase activity; IDA:HGNC.
GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
GO:0033523; P:histone H2B ubiquitination; IDA:UniProtKB.
GO:0010390; P:histone monoubiquitination; IDA:UniProtKB.
GO:0032020; P:ISG15-protein conjugation; IDA:HGNC.
GO:0007094; P:mitotic spindle assembly checkpoint; TAS:Reactome.
GO:0051436; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
GO:0051437; P:positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
GO:0070936; P:protein K48-linked ubiquitination; IDA:UniProtKB.

IPR000608; UBQ-conjugat_E2.
IPR023313; UBQ-conjugating_AS.
IPR016135; UBQ-conjugating_enzyme/RWD.

PS00183; UBIQUITIN_CONJUGAT_1
PS50127; UBIQUITIN_CONJUGAT_2