CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007087
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA damage response protein kinase DUN1 
Protein Synonyms/Alias
  
Gene Name
 DUN1 
Gene Synonyms/Alias
 YDL101C; D2370 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
151DIENDDEKVSSESRSubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Transducer of the DNA damage signal. Phosphorylates SML1 on serine residues. Cooperates with the PAN deadenylation complex in the regulation of RAD5 mRNA levels and cell survival in response to replicational stress. 
Sequence Annotation
 DOMAIN 56 112 FHA.
 DOMAIN 200 480 Protein kinase.
 NP_BIND 206 214 ATP (By similarity).
 ACT_SITE 328 328 Proton acceptor.
 BINDING 229 229 ATP.
 MOD_RES 10 10 Phosphoserine.
 MOD_RES 139 139 Phosphoserine.
 MOD_RES 380 380 Phosphothreonine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; DNA damage; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 513 AA 
Protein Sequence
MSLSTKREHS GDVTDSSFKR QQRSNKPSSE YTCLGHLVNL IPGKEQKVEI TNRNVTTIGR 60
SRSCDVILSE PDISTFHAEF HLLQMDVDNF QRNLINVIDK SRNGTFINGN RLVKKDYILK 120
NGDRIVFGKS CSFLFKYASS SSTDIENDDE KVSSESRSYK NDDEVFKKPQ ISATSSQNAT 180
TSAAIRKLNK TRPVSFFDKY LLGKELGAGH YALVKEAKNK KTGQQVAVKI FHAQQNDDQK 240
KNKQFREETN ILMRVQHPNI VNLLDSFVEP ISKSQIQKYL VLEKIDDGEL FERIVRKTCL 300
RQDESKALFK QLLTGLKYLH EQNIIHRDIK PENILLNITR RENPSQVQLG PWDEDEIDIQ 360
VKIADFGLAK FTGEMQFTNT LCGTPSYVAP EVLTKKGYTS KVDLWSAGVI LYVCLCGFPP 420
FSDQLGPPSL KEQILQAKYA FYSPYWDKID DSVLHLISNL LVLNPDERYN IDEALNHPWF 480
NDIQQQSSVS LELQRLQITD NKIPKTYSEL SCL 513 
Gene Ontology
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; IDA:SGD.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0000077; P:DNA damage checkpoint; IMP:SGD.
 GO:0048478; P:replication fork protection; IGI:SGD. 
Interpro
 IPR000253; FHA_dom.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS.
 IPR008984; SMAD_FHA_domain. 
Pfam
 PF00498; FHA
 PF00069; Pkinase 
SMART
 SM00240; FHA
 SM00220; S_TKc 
PROSITE
 PS50006; FHA_DOMAIN
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS