CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018810
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras-responsive element-binding protein 1 
Protein Synonyms/Alias
 RREB-1; Finger protein in nuclear bodies; Raf-responsive zinc finger protein LZ321; Zinc finger motif enhancer-binding protein 1; Zep-1 
Gene Name
 RREB1 
Gene Synonyms/Alias
 FINB 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
435PATKDSIKHLSLQPFubiquitination[1]
615LSMEAKIKQEITEGEsumoylation[2]
802CSAAFAAKRNCIHHIubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.
 Matic I, Schimmel J, Hendriks IA, van Santen MA, van de Rijke F, van Dam H, Gnad F, Mann M, Vertegaal AC.
 Mol Cell. 2010 Aug 27;39(4):641-52. [PMID: 20797634]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Transcription factor that binds specifically to the RAS- responsive elements (RRE) of gene promoters. May be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. Represses the angiotensinogen gene. Negatively regulates the transcriptional activity of AR. Potentiates the transcriptional activity of NEUROD1. 
Sequence Annotation
 ZN_FING 66 88 C2H2-type 1.
 ZN_FING 97 119 C2H2-type 2.
 ZN_FING 125 147 C2H2-type 3.
 ZN_FING 208 230 C2H2-type 4.
 ZN_FING 235 258 C2H2-type 5.
 ZN_FING 316 338 C2H2-type 6.
 ZN_FING 643 665 C2H2-type 7.
 ZN_FING 671 693 C2H2-type 8.
 ZN_FING 699 722 C2H2-type 9.
 ZN_FING 753 775 C2H2-type 10.
 ZN_FING 790 815 C2H2-type 11.
 ZN_FING 1246 1268 C2H2-type 12.
 ZN_FING 1393 1415 C2H2-type 13.
 ZN_FING 1512 1534 C2H2-type 14.
 ZN_FING 1540 1562 C2H2-type 15.
 MOD_RES 36 36 Phosphoserine.
 MOD_RES 42 42 Phosphoserine.
 MOD_RES 161 161 Phosphoserine.
 MOD_RES 231 231 Phosphoserine.
 MOD_RES 1122 1122 Phosphoserine.
 MOD_RES 1134 1134 Phosphoserine (By similarity).
 MOD_RES 1135 1135 Phosphoserine (By similarity).
 MOD_RES 1167 1167 Phosphoserine.
 MOD_RES 1174 1174 Phosphoserine.
 MOD_RES 1175 1175 Phosphoserine.
 MOD_RES 1219 1219 Phosphoserine.
 MOD_RES 1225 1225 Phosphoserine.
 MOD_RES 1315 1315 Phosphothreonine.
 MOD_RES 1320 1320 Phosphoserine.
 MOD_RES 1475 1475 Phosphoserine.
 MOD_RES 1653 1653 Phosphoserine.  
Keyword
 Activator; Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1687 AA 
Protein Sequence
MTSSSPAGLE GSDLSSINTM MSAVMSVGKV TENGGSPQGI KSPSKPPGPN RIGRRNQETK 60
EEKSSYNCPL CEKICTTQHQ LTMHIRQHNT DTGGADHSCS ICGKSLSSAS SLDRHMLVHS 120
GERPYKCTVC GQSFTTNGNM HRHMKIHEKD PNSATATAPP SPLKRRRLSS KRKLSHDAES 180
EREDPAPAKK MVEDGQSGDL EKKADEVFHC PVCFKEFVCK YGLETHMETH SDNPLRCDIC 240
CVTFRTHRGL LRHNALVHKQ LPRDAMGRPF IQNNPSIPAG FHDLGFTDFS CRKFPRISQA 300
WCETNLRRCI SEQHRFVCDT CDKAFPMLCS LALHKQTHVA ADQGQEKPQA TPLPGDALDQ 360
KGFLALLGLQ HTKDVRPAPA EEPLPDDNQA IQLQTLKCQL PQDPGCTNLL SLSPFEAASL 420
GGSLTVLPAT KDSIKHLSLQ PFQKGFIIQP DSSIVVKPIS GESAIELADI QQILKMAASA 480
PPQISLPPFS KAPAAPLQAI FKHMPPLKPK PLVTPRTVVA TSTPPPLINA QQASPGCISP 540
SLPPPPLKLL KGSVEAASNA HLLQSKSGTQ PHAATRLSLQ QPRAELPGQP EMKTQLEQDS 600
IIEALLPLSM EAKIKQEITE GELKAFMTAP GGKKTPAMRK VLYPCRFCNQ VFAFSGVLRA 660
HVRSHLGISP YQCNICDYIA ADKAALIRHL RTHSGERPYI CKICHYPFTV KANCERHLRK 720
KHLKATRKDI EKNIEYVSSS AAELVDAFCA PDTVCRLCGE DLKHYRALRI HMRTHCGRGL 780
GGGHKGRKPF ECKECSAAFA AKRNCIHHIL KQHLHVPEQD IESYVLAADG LGPAEAPAAE 840
ASGRGEDSGC AALGDCKPLT AFLEPQNGFL HRGPTQPPPP HVSIKLEPAS SFAVDFNEPL 900
DFSQKGLALV QVKQENISFL SPSSLVPYDC SMEPIDLSIP KNFRKGDKDL ATPSEAKKPE 960
EEAGSSEQPS PCPAPGPSLP VTLGPSGILE SPMAPAPAAT PEPPAQPLQG PVQLAVPIYS 1020
SALVSSPPLV GSSALLSGTA LLRPLRPKPP LLLPKPPVTE ELPPLASIAQ IISSVSSAPT 1080
LLKTKVADPG PASTGSNTTA SDSLGGSVPK AATTATPAAT TSPKESSEPP APASSPEAAS 1140
PTEQGPAGTS KKRGRKRGMR SRPRANSGGV DLDSSGEFAS IEKMLATTDT NKFSPFLQTA 1200
EDNTQDEVAG APADHHGPSD EEQGSPPEDK LLRAKRNSYT NCLQKITCPH CPRVFPWASS 1260
LQRHMLTHTD SQSDAETAAA AGEVLDLTSR DREQPSEGAT ELRQVAGDAP VEQATAETAS 1320
PVHREEHGRG ESHEPEEEHG TEESTGDADG AEEDASSNQS LDLDFATKLM DFKLAEGDGE 1380
AGAGGAASQE QKLACDTCGK SFKFLGTLSR HRKAHGRQEP KDEKGDGAST AEEGPQPAPE 1440
QEEKPPETPA EVVESAPGAG EAPAEKLAEE TEGPSDGESA AEKRSSEKSD DDKKPKTDSP 1500
KSVASKADKR KKVCSVCNKR FWSLQDLTRH MRSHTGERPY KCQTCERTFT LKHSLVRHQR 1560
IHQKARHAKH HGKDSDKEER GEEDSENEST HSGNNAVSEN EAELAPNASN HMAVTRSRKE 1620
GLASATKDCS HREEKVTAGW PSEPGQGDLN PESPAALGQD LLEPRSKRPA HPILATADGA 1680
SQLVGME 1687 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:UniProtKB.
 GO:0016604; C:nuclear body; TAS:UniProtKB.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007275; P:multicellular organismal development; TAS:ProtInc.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; TAS:UniProtKB.
 GO:0007265; P:Ras protein signal transduction; TAS:ProtInc.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF00096; zf-C2H2 
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS