UniProt Accession

 MDHM_YEAST; P17505; D6VXK3; E9P8U0 

Genbank Protein ID

 J02841; Z28085; AY557914; BK006944 

Genbank Nucleotide ID

 AAA34759.1; CAA81923.1; AAS56240.1; DAA09073.1 

Protein Name

 Malate dehydrogenase, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 MDH1 

Gene Synonyms/Alias

 YKL085W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
233	FGGDEVVKAKNGAGS	acetylation	[1]
261	NAVLSGFKGERDVIE	acetylation	[1]
278	FVDSPLFKSEGIEFF	ubiquitination	[2]
326	TLKKNIEKGVNFVAS	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

  

Sequence Annotation

 NP_BIND 24 30 NAD (By similarity).
 NP_BIND 135 137 NAD (By similarity).
 ACT_SITE 195 195 Proton acceptor (By similarity).
 BINDING 50 50 NAD (By similarity).
 BINDING 99 99 Substrate (By similarity).
 BINDING 105 105 Substrate (By similarity).
 BINDING 112 112 NAD (By similarity).
 BINDING 137 137 Substrate (By similarity).
 BINDING 171 171 Substrate (By similarity).
 BINDING 245 245 NAD (By similarity).
 MOD_RES 177 177 Phosphoserine.
 MOD_RES 199 199 Phosphothreonine.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 334 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IDA:SGD.
 GO:0030060; F:L-malate dehydrogenase activity; IDA:SGD.
 GO:0003729; F:mRNA binding; IDA:SGD.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0001300; P:chronological cell aging; IMP:SGD.
 GO:0006108; P:malate metabolic process; IEA:InterPro.
 GO:0001302; P:replicative cell aging; IMP:SGD.
 GO:0006099; P:tricarboxylic acid cycle; IMP:SGD. 

Interpro

 IPR001557; L-lactate/malate_DH.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR001252; Malate_DH_AS.
 IPR010097; Malate_DH_type1.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 

SMART

  

PROSITE

 PS00068; MDH 

PRINTS