CPLM-019332

Genbank Nucleotide ID

Far upstream element-binding protein 3

Protein Synonyms/Alias

FUSE-binding protein 3

Homo sapiens (Human)

Position	Peptide	Type	References
15	QSAPVGMKAEGFVDA	ubiquitination	[1, 2, 3]
32	RVRQIAAKIDSIPHL	acetylation	[4]
32	RVRQIAAKIDSIPHL	ubiquitination	[2]
57	VYGYGVQKRPLDDGV	ubiquitination	[2, 3, 5, 6]
83	TVITEEFKVPDKMVG	ubiquitination	[2]
87	EEFKVPDKMVGFIIG	ubiquitination	[2, 3, 5, 6, 7]
138	PESIEQAKRLLGQIV	ubiquitination	[2]
186	GRGGETIKQLQERTG	ubiquitination	[2, 8]
195	LQERTGVKMVMIQDG	ubiquitination	[2]
220	RITGDAFKVQQAREM	ubiquitination	[2, 3]
378	GKGGENIKSINQQSG	ubiquitination	[3, 5, 6, 7]
525	KAWEDYYKKQSHAAS	ubiquitination	[1, 5, 6, 7]
526	AWEDYYKKQSHAASA	ubiquitination	[1, 2, 3]

[1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

May interact with single-stranded DNA from the far- upstream element (FUSE). May activate gene expression.

DOMAIN 77 141 KH 1.
DOMAIN 162 228 KH 2.
DOMAIN 253 317 KH 3.
DOMAIN 354 421 KH 4.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 76 76 Phosphothreonine.
MOD_RES 296 296 Phosphoserine.
MOD_RES 539 539 Phosphoserine.
MOD_RES 569 569 Phosphoserine.

Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IDA:UniProtKB.
GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO:0003723; F:RNA binding; IEA:InterPro.
GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
GO:0006351; P:transcription, DNA-dependent; IDA:UniProtKB.

IPR004087; KH_dom.
IPR004088; KH_dom_type_1.