UniProt Accession

 B4E363_HUMAN; B4E363 

Genbank Protein ID

 AC092069; AK304587 

Genbank Nucleotide ID

 BAG65375.1 

Protein Name

 Phenylalanine--tRNA ligase alpha subunit 

Protein Synonyms/Alias

 cDNA FLJ50378, highly similar to Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20) 

Gene Name

 FARSA 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
59	EAELRSTKHWELTAE	ubiquitination	[1, 2, 3, 4, 5]
101	LMRLPSGKVGFSKAM	ubiquitination	[1, 2, 3, 4, 6]
106	SGKVGFSKAMSNKWI	ubiquitination	[1, 2, 3, 4, 6]
111	FSKAMSNKWIRVDKS	ubiquitination	[2, 3, 4, 6]
117	NKWIRVDKSAADGPR	acetylation	[6]
117	NKWIRVDKSAADGPR	ubiquitination	[1, 4, 6]
150	VRGGQAEKLGEKERS	ubiquitination	[1, 2, 4]
154	QAEKLGEKERSELRK	ubiquitination	[4]
177	SWRDRPFKPYNFLAH	ubiquitination	[2, 4, 6]
198	GHLHPLLKVRSQFRQ	ubiquitination	[2]
280	GYGSQGYKYNWKLDE	ubiquitination	[1, 2, 3, 4, 5, 6]
284	QGYKYNWKLDEARKN	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
311	ALYRLAQKKPFTPVK	ubiquitination	[2, 4, 6]
312	LYRLAQKKPFTPVKY	ubiquitination	[1, 2, 4, 6]
318	KKPFTPVKYFSIDRV	ubiquitination	[2, 4, 5, 7]
366	VLREFFTKLGITQLR	ubiquitination	[1, 2, 4]
375	GITQLRFKPAYNPYT	ubiquitination	[1, 2, 4, 6, 7]
396	FSYHQGLKKWVEVGN	ubiquitination	[2, 3, 4, 5]
397	SYHQGLKKWVEVGNS	ubiquitination	[4]
437	LERPTMIKYGINNIR	ubiquitination	[7]
450	IRELVGHKVNLQMVY	ubiquitination	[2, 3, 4, 6, 7]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

  

Sequence Annotation

  

Keyword

 Aminoacyl-tRNA synthetase; Complete proteome; Ligase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 477 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004826; F:phenylalanine-tRNA ligase activity; IEA:InterPro.
 GO:0000049; F:tRNA binding; IEA:InterPro.
 GO:0006432; P:phenylalanyl-tRNA aminoacylation; IEA:InterPro. 

Interpro

 IPR006195; aa-tRNA-synth_II.
 IPR004529; Phe-tRNA-synth_IIc_asu.
 IPR002319; Phenylalanyl-tRNA_Synthase.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF01409; tRNA-synt_2d 

SMART

  

PROSITE

 PS50862; AA_TRNA_LIGASE_II 

PRINTS