CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014396
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 
Protein Synonyms/Alias
 Phosphoinositide phospholipase C-gamma-1; Phospholipase C-gamma-1; PLC-gamma-1 
Gene Name
 Plcg1 
Gene Synonyms/Alias
 Plcg-1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
178RISAKDLKNMLSQVNubiquitination[1]
226SLMYSAQKTMDLPFLubiquitination[1]
431NMAQHFRKVLGDTLLubiquitination[1]
440LGDTLLTKPVDIAADubiquitination[1]
1215KIDIFPAKENGDLSPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand- mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration. 
Sequence Annotation
 DOMAIN 27 142 PH 1.
 DOMAIN 152 187 EF-hand.
 DOMAIN 320 464 PI-PLC X-box.
 DOMAIN 489 523 PH 2; first part.
 DOMAIN 550 657 SH2 1.
 DOMAIN 668 756 SH2 2.
 DOMAIN 791 851 SH3.
 DOMAIN 895 931 PH 2; second part.
 DOMAIN 953 1070 PI-PLC Y-box.
 DOMAIN 1075 1177 C2.
 ACT_SITE 335 335 By similarity.
 ACT_SITE 380 380 By similarity.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 379 379 Phosphotyrosine.
 MOD_RES 481 481 Phosphotyrosine.
 MOD_RES 506 506 Phosphotyrosine.
 MOD_RES 771 771 Phosphotyrosine; by SYK.
 MOD_RES 775 775 Phosphotyrosine.
 MOD_RES 783 783 Phosphotyrosine; by ITK, SYK and TXK (By
 MOD_RES 977 977 Phosphotyrosine.
 MOD_RES 1221 1221 Phosphoserine (By similarity).
 MOD_RES 1248 1248 Phosphoserine.
 MOD_RES 1253 1253 Phosphotyrosine (By similarity).
 MOD_RES 1263 1263 Phosphoserine.  
Keyword
 Acetylation; Calcium; Cell projection; Complete proteome; Hydrolase; Lipid degradation; Lipid metabolism; Metal-binding; Phosphoprotein; Reference proteome; Repeat; SH2 domain; SH3 domain; Transducer; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1302 AA 
Protein Sequence
MAGVATPCAN GCGPGAPSEA EVLHLCRSLE VGTVMTLFYS KKSQRPERKT FQVKLETRQI 60
TWSRGADKIE GSIDIREIKE IRPGKTSRDF DRYQEDPAFR PDQSHCFVIL YGMEFRLKTL 120
SLQATSEDEV NMWIKGLTWL MEDTLQAATP LQIERWLRKQ FYSVDRNRED RISAKDLKNM 180
LSQVNYRVPN MRFLRERLTD LEQRSGDITY GQFAQLYRSL MYSAQKTMDL PFLETNALRT 240
GERPEHCQVS LSEFQQFLLE YQGELWAVDR LQVQEFMLSF LRDPLREIEE PYFFLDELVT 300
FLFSKENSVW NSQLDAVCPD TMNNPLSHYW ISSSHNTYLT GDQFSSESSL EAYARCLRMG 360
CRCIELDCWD GPDGMPVIYH GHTLTTKIKF SDVLHTIKEH AFVASEYPVI LSIEDHCSIA 420
QQRNMAQHFR KVLGDTLLTK PVDIAADGLP SPNQLRRKIL IKHKKLAEGS AYEEVPTSVM 480
YSENDISNSI KNGILYLEDP VNHEWYPHYF VLTSSKIYYS EETSSDQGNE DEEEPKEASS 540
STELHSSEKW FHGKLGAGRD GRHIAERLLT EYCIETGAPD GSFLVRESET FVGDYTLSFW 600
RNGKVQHCRI HSRQDAGTPK FFLTDNLVFD SLYDLITHYQ QVPLRCNEFE MRLSEPVPQT 660
NAHESKEWYH ASLTRAQAEH MLMRVPRDGA FLVRKRNEPN SYAISFRAEG KIKHCRVQQE 720
GQTVMLGNSE FDSLVDLISY YEKHPLYRKM KLRYPINEEA LEKIGTAEPD YGALYEGRNP 780
GFYVEANPMP TFKCAVKALF DYKAQREDEL TFTKSAIIQN VEKQDGGWWR GDYGGKKQLW 840
FPSNYVEEMI NPAVLEPERE HLDENSPLGD LLRGVLDVPA CQIAIRPEGK NNRLFVFSIS 900
MPSVAQWSLD VAADSQEELQ DWVKKIREVA QTADARLTEG KMMERRKKIA LELSELVVYC 960
RPVPFDEEKI GTERACYRDM SSFPETKAEK YVNKAKGKKF LQYNRLQLSR IYPKGQRLDS 1020
SNYDPLPMWI CGSQLVALNF QTPDKPMQMN QALFMAGGHC GYVLQPSTMR DEAFDPFDKS 1080
SLRGLEPCVI CIEVLGARHL PKNGRGIVCP FVEIEVAGAE YDSTKQKTEF VVDNGLNPVW 1140
PAKPFHFQIS NPEFAFLRFV VYEEDMFSDQ NFLAQATFPV KGLKTGYRAV PLKNNYSEDL 1200
ELASLLIKID IFPAKENGDL SPFSGISLRE RASDASSQLF HVRAREGSFE ARYQQPFEDF 1260
RISQEHLADH FDSRERSTSD GPSSATNLIE DPLHDKLWKC SL 1302 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030027; C:lamellipodium; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0001726; C:ruffle; ISS:UniProtKB.
 GO:0008180; C:signalosome; IEA:Compara.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0004435; F:phosphatidylinositol phospholipase C activity; IEA:EC.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0004871; F:signal transducer activity; IEA:UniProtKB-KW.
 GO:0071364; P:cellular response to epidermal growth factor stimulus; ISS:UniProtKB.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0009395; P:phospholipid catabolic process; IEA:InterPro.
 GO:0045766; P:positive regulation of angiogenesis; IEA:Compara.
 GO:0043536; P:positive regulation of blood vessel endothelial cell migration; IEA:Compara.
 GO:0010634; P:positive regulation of epithelial cell migration; ISS:UniProtKB.
 GO:0050852; P:T cell receptor signaling pathway; IDA:MGI. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR011993; PH_like_dom.
 IPR001192; Pinositol_PLipase_C.
 IPR016279; PLC-gamma.
 IPR017946; PLC-like_Pdiesterase_TIM-brl.
 IPR001849; Pleckstrin_homology.
 IPR015359; PLipase_C_EF-hand-like.
 IPR000909; PLipase_C_PInositol-sp_X_dom.
 IPR001711; PLipase_C_Pinositol-sp_Y.
 IPR000980; SH2.
 IPR001452; SH3_domain. 
Pfam
 PF00168; C2
 PF09279; efhand_like
 PF00169; PH
 PF00388; PI-PLC-X
 PF00387; PI-PLC-Y
 PF00017; SH2
 PF00018; SH3_1 
SMART
 SM00239; C2
 SM00233; PH
 SM00148; PLCXc
 SM00149; PLCYc
 SM00252; SH2
 SM00326; SH3 
PROSITE
 PS50004; C2
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50003; PH_DOMAIN
 PS50007; PIPLC_X_DOMAIN
 PS50008; PIPLC_Y_DOMAIN
 PS50001; SH2
 PS50002; SH3 
PRINTS
 PR00390; PHPHLIPASEC.
 PR00401; SH2DOMAIN.
 PR00452; SH3DOMAIN.