CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021642
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calpain-10 
Protein Synonyms/Alias
 Calcium-activated neutral proteinase 10; CANP 10 
Gene Name
 CAPN10 
Gene Synonyms/Alias
 KIAA1845 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
188LAERWNLKGVAGSGGubiquitination[1]
348ALPGAWVKGQSAGGCubiquitination[1]
501SAIRAVAKNTTPGAAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. 
Sequence Annotation
 DOMAIN 13 321 Calpain catalytic.
 REGION 322 494 Domain III 1.
 REGION 513 654 Domain III 2.
 ACT_SITE 73 73 By similarity.
 ACT_SITE 238 238 By similarity.
 ACT_SITE 263 263 By similarity.  
Keyword
 Alternative splicing; Complete proteome; Diabetes mellitus; Hydrolase; Polymorphism; Protease; Reference proteome; Repeat; Thiol protease. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 672 AA 
Protein Sequence
MRAGRGATPA RELFRDAAFP AADSSLFCDL STPLAQFRED ITWRRPQEIC ATPRLFPDDP 60
REGQVKQGLL GDCWFLCACA ALQKSRHLLD QVIPPGQPSW ADQEYRGSFT CRIWQFGRWV 120
EVTTDDRLPC LAGRLCFSRC QREDVFWLPL LEKVYAKVHG SYEHLWAGQV ADALVDLTGG 180
LAERWNLKGV AGSGGQQDRP GRWEHRTCRQ LLHLKDQCLI SCCVLSPRAG ARELGEFHAF 240
IVSDLRELQG QAGQCILLLR IQNPWGRRCW QGLWREGGEG WSQVDAAVAS ELLSQLQEGE 300
FWVEEEEFLR EFDELTVGYP VTEAGHLQSL YTERLLCHTR ALPGAWVKGQ SAGGCRNNSG 360
FPSNPKFWLR VSEPSEVYIA VLQRSRLHAA DWAGRARALV GDSHTSWSPA SIPGKHYQAV 420
GLHLWKVEKR RVNLPRVLSM PPVAGTACHA YDREVHLRCE LSPGYYLAVP STFLKDAPGE 480
FLLRVFSTGR VSLSAIRAVA KNTTPGAALP AGEWGTVQLR GSWRVGQTAG GSRNFASYPT 540
NPCFPFSVPE GPGPRCVRIT LHQHCRPSDT EFHPIGFHIF QVPEGGRSQD APPLLLQEPL 600
LSCVPHRYAQ EVSRLCLLPA GTYKVVPSTY LPDTEGAFTV TIATRIDRPS IHSQEMLGQF 660
LQEVSIMAVM KT 672 
Gene Ontology
 GO:0005829; C:cytosol; ISS:BHF-UCL.
 GO:0005886; C:plasma membrane; ISS:BHF-UCL.
 GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IMP:BHF-UCL.
 GO:0008092; F:cytoskeletal protein binding; ISS:BHF-UCL.
 GO:0000149; F:SNARE binding; ISS:BHF-UCL.
 GO:0031532; P:actin cytoskeleton reorganization; ISS:BHF-UCL.
 GO:0032869; P:cellular response to insulin stimulus; IMP:BHF-UCL.
 GO:0097194; P:execution phase of apoptosis; IDA:BHF-UCL.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0046326; P:positive regulation of glucose import; IMP:BHF-UCL.
 GO:0032024; P:positive regulation of insulin secretion; IMP:BHF-UCL.
 GO:0032388; P:positive regulation of intracellular transport; ISS:BHF-UCL.
 GO:0006508; P:proteolysis; IMP:BHF-UCL.
 GO:0097050; P:type B pancreatic cell apoptotic process; IDA:BHF-UCL. 
Interpro
 IPR022684; Calpain_cysteine_protease.
 IPR022682; Calpain_domain_III.
 IPR022683; Calpain_III.
 IPR000169; Pept_cys_AS.
 IPR001300; Peptidase_C2_calpain_cat. 
Pfam
 PF01067; Calpain_III
 PF00648; Peptidase_C2 
SMART
 SM00720; calpain_III
 SM00230; CysPc 
PROSITE
 PS50203; CALPAIN_CAT
 PS00640; THIOL_PROTEASE_ASN
 PS00139; THIOL_PROTEASE_CYS
 PS00639; THIOL_PROTEASE_HIS 
PRINTS
 PR00704; CALPAIN.