UniProt Accession

 ERF1_HUMAN; P62495; B2R6B4; D3DQC1; P46055; Q5M7Z7 

Genbank Protein ID

 M75715; X81625; U90176; AF095901; AK312510; CH471062; CH471062; BC088358 

Genbank Nucleotide ID

 AAA36665.1; CAA57281.1; AAB49726.1; AAD43966.1; BAG35411.1; EAW62130.1; EAW62131.1; AAH88358.1 

Protein Name

 Eukaryotic peptide chain release factor subunit 1 

Protein Synonyms/Alias

 Eukaryotic release factor 1; eRF1; Protein Cl1; TB3-1 

Gene Name

 ETF1 

Gene Synonyms/Alias

 ERF1; RF1; SUP45L1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
171	NTREVLHKFTVDLPK	ubiquitination	[1, 2, 3]
384	WFANNYKKFGATLEI	ubiquitination	[1, 3, 4, 5]
395	TLEIVTDKSQEGSQF	ubiquitination	[2, 4, 6]
404	QEGSQFVKGFGGIGG	ubiquitination	[1, 3, 4, 5, 6]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Nonsense-mediated mRNA decay; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 437 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0043022; F:ribosome binding; TAS:UniProtKB.
 GO:0003747; F:translation release factor activity; TAS:UniProtKB.
 GO:0016149; F:translation release factor activity, codon specific; IEA:InterPro.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
 GO:0006479; P:protein methylation; IDA:MGI.
 GO:0006449; P:regulation of translational termination; TAS:ProtInc. 

Interpro

 IPR005140; eRF1_1_Pelota.
 IPR005141; eRF1_2.
 IPR005142; eRF1_3.
 IPR004403; Peptide_chain-rel_eRF1/aRF1.
 IPR024049; Release_factor_eRF1/aRF1_N. 

Pfam

 PF03463; eRF1_1
 PF03464; eRF1_2
 PF03465; eRF1_3 

SMART

  

PROSITE

  

PRINTS