CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012216
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 General transcription factor IIH subunit 3 
Protein Synonyms/Alias
 Basic transcription factor 2 34 kDa subunit; BTF2 p34; General transcription factor IIH polypeptide 3; TFIIH basal transcription factor complex p34 subunit 
Gene Name
 GTF2H3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
28WWGKQALKESQFTLSubiquitination[1]
56LFMNRSNKLAVIASHubiquitination[2]
74SRFLYPGKNGRLGDFubiquitination[1]
97EFNPSGSKDGKYELLubiquitination[2, 3]
100PSGSKDGKYELLTSAubiquitination[3]
121EIKDLMTKSDIKGQHubiquitination[3]
125LMTKSDIKGQHTETLubiquitination[1, 2, 3]
152HRMNKEVKDNQEMKSubiquitination[3]
158VKDNQEMKSRILVIKubiquitination[3]
298ISLPPVLKAKKKKLKubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Anchors XPB. 
Sequence Annotation
 ZN_FING 268 285 C4-type.  
Keyword
 Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Metal-binding; Nucleus; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 308 AA 
Protein Sequence
MVSDEDELNL LVIVVDANPI WWGKQALKES QFTLSKCIDA VMVLGNSHLF MNRSNKLAVI 60
ASHIQESRFL YPGKNGRLGD FFGDPGNPPE FNPSGSKDGK YELLTSANEV IVEEIKDLMT 120
KSDIKGQHTE TLLAGSLAKA LCYIHRMNKE VKDNQEMKSR ILVIKAAEDS ALQYMNFMNV 180
IFAAQKQNIL IDACVLDSDS GLLQQACDIT GGLYLKVPQM PSLLQYLLWV FLPDQDQRSQ 240
LILPPPVHVD YRAACFCHRN LIEIGYVCSV CLSIFCNFSP ICTTCETAFK ISLPPVLKAK 300
KKKLKVSA 308 
Gene Ontology
 GO:0000439; C:core TFIIH complex; IEA:InterPro.
 GO:0005675; C:holo TFIIH complex; IDA:UniProtKB.
 GO:0003684; F:damaged DNA binding; NAS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:UniProtKB.
 GO:0008135; F:translation factor activity, nucleic acid binding; TAS:ProtInc.
 GO:0006370; P:7-methylguanosine mRNA capping; TAS:Reactome.
 GO:0000718; P:nucleotide-excision repair, DNA damage removal; TAS:Reactome.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0006468; P:protein phosphorylation; IDA:GOC.
 GO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome.
 GO:0006362; P:transcription elongation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR004600; TFIIH_Tfb4/p34. 
Pfam
 PF03850; Tfb4 
SMART
  
PROSITE
  
PRINTS