| Tag | Content |
|---|
| CPLM ID | CPLM-000513 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB |
Protein Synonyms/Alias | tRNA-i(6)A37 methylthiotransferase |
Gene Name | miaB |
Gene Synonyms/Alias | ymcB; BSU17010 |
Created Date | July 27, 2013 |
Organism | Bacillus subtilis (strain 168) |
NCBI Taxa ID | 224308 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 258 | RLASEGYKEITLLGQ | acetylation | [1] |
|
Reference | [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis. Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG. Proteomics. 2013 May;13(10-11):1726-36. [ PMID: 23468065] |
Functional Description | Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine. |
Sequence Annotation | DOMAIN 66 184 MTTase N-terminal.
DOMAIN 440 503 TRAM.
METAL 75 75 Iron-sulfur (4Fe-4S) (By similarity).
METAL 111 111 Iron-sulfur (4Fe-4S) (By similarity).
METAL 145 145 Iron-sulfur (4Fe-4S) (By similarity).
METAL 221 221 Iron-sulfur (4Fe-4S-S-AdoMet) (By
METAL 225 225 Iron-sulfur (4Fe-4S-S-AdoMet) (By
METAL 228 228 Iron-sulfur (4Fe-4S-S-AdoMet) (By |
Keyword | 4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur; Metal-binding; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 509 AA |
Protein Sequence | MNEKQKLESG QVHPSDKKSE KDYSKYFEAV YIPPSLKDAK KRGKEAVTYH NDFKISEQFK 60
GLGDGRKFYI RTYGCQMNEH DTEVMAGIFM ALGYEATNSV DDANVILLNT CAIRENAENK 120
VFGELGHLKA LKKNNPDLIL GVCGCMSQEE SVVNRILKKH PFVDMIFGTH NIHRLPELLS 180
EAYLSKEMVV EVWSKEGDVI ENLPKVRNGK IKGWVNIMYG CDKFCTYCIV PYTRGKERSR 240
RPEDIIQEVR RLASEGYKEI TLLGQNVNAY GKDFEDMTYG LGDLMDELRK IDIPRIRFTT 300
SHPRDFDDRL IEVLAKGGNL LDHIHLPVQS GSSEVLKLMA RKYDRERYME LVRKIKEAMP 360
NASLTTDIIV GFPNETDEQF EETLSLYREV EFDSAYTFIY SPREGTPAAK MKDNVPMRVK 420
KERLQRLNAL VNEISAKKMK EYEGKVVEVL VEGESKNNPD ILAGYTEKSK LVNFKGPKEA 480
IGKIVRVKIQ QAKTWSLDGE MVGEAIEVK 509 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:HAMAP. GO:0005506; F:iron ion binding; IEA:HAMAP. GO:0035597; F:N6-isopentenyladenosine methylthiotransferase activity; IMP:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |