CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005705
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CUGBP Elav-like family member 1 
Protein Synonyms/Alias
 CELF-1; 50 kDa nuclear polyadenylated RNA-binding protein; Brain protein F41; Bruno-like protein 2; CUG triplet repeat RNA-binding protein 1; CUG-BP1; CUG-BP- and ETR-3-like factor 1; Deadenylation factor CUG-BP; Deadenylation factor EDEN-BP; Embryo deadenylation element-binding protein homolog; EDEN-BP homolog; RNA-binding protein BRUNOL-2 
Gene Name
 Celf1 
Gene Synonyms/Alias
 Brunol2; Cugbp; Cugbp1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
44QPDLDAIKMFVGQVPubiquitination[1]
57VPRTWSEKDLRELFEubiquitination[1]
97FVTFYTRKAALEAQNubiquitination[1]
193TMAQTAIKAMHQAQTubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing (By similarity). Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition (By similarity). Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs (By similarity). Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB (By similarity). Promotes exclusion of exon 11 of the INSR pre-mRNA (By similarity). Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast (By similarity). Increases translation and controls the choice of translation initiation codon of CEBPB mRNA (By similarity). Increases mRNA translation of CEBPB in aging liver. Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3 (By similarity). Mediates rapid cytoplasmic mRNA deadenylation (By similarity). Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation (By similarity). Required for completion of spermatogenesis. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs) (By similarity). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity). Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA (By similarity). Binds to the 5'-region of CDKN1A and CEBPB mRNAs (By similarity). Binds with the 5'-region of CEBPB mRNA in aging liver. 
Sequence Annotation
 DOMAIN 16 99 RRM 1.
 DOMAIN 108 188 RRM 2.
 DOMAIN 401 479 RRM 3.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 302 302 Phosphoserine.  
Keyword
 Acetylation; Activator; Alternative splicing; Complete proteome; Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 486 AA 
Protein Sequence
MAAFKLDFLP EMMVDHCSLN SSPVSKKMNG TLDHPDQPDL DAIKMFVGQV PRTWSEKDLR 60
ELFEQYGAVY EINILRDRSQ NPPQSKGCCF VTFYTRKAAL EAQNALHNMK VLPGMHHPIQ 120
MKPADSEKNN AVEDRKLFIG MISKKCTEND IRVMFSSFGQ IEECRILRGP DGLSRGCAFV 180
TFTTRTMAQT AIKAMHQAQT MEGCSSPMVV KFADTQKDKE QKRMAQQLQQ QMQQISAASV 240
WGNLAGLNTL GPQYLALYLQ LLQQTASSGN LNTLSSLHPM GGLNAMQLQN LAALAAAASA 300
AQNTPSGTNA LTTSSSPLSV LTSSGSSPSS SSSNSVNPIA SLGALQTLAG ATAGLNVGSL 360
AGMAALNGGL GSSGLSNGTG STMEALTQAY SGIQQYAAAA LPTLYNQNLL TQQSIGAAGS 420
QKEGPEGANL FIYHLPQEFG DQDLLQMFMP FGNVVSAKVF IDKQTNLSKC FGFVSYDNPV 480
SAQAAIQSMN GFQIGMKRLK VQLKRSKNDS KPY 513 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0042835; F:BRE binding; ISS:UniProtKB.
 GO:0003729; F:mRNA binding; IEA:Compara.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0031369; F:translation initiation factor binding; IDA:MGI.
 GO:0006376; P:mRNA splice site selection; IDA:MGI.
 GO:0040018; P:positive regulation of multicellular organism growth; IMP:MGI.
 GO:0043484; P:regulation of RNA splicing; ISS:UniProtKB.
 GO:0007286; P:spermatid development; IMP:MGI. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
 PF00076; RRM_1 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS