CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019731
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitogen-activated protein kinase kinase kinase 14 
Protein Synonyms/Alias
 NF-kappa-beta-inducing kinase; HsNIK; Serine/threonine-protein kinase NIK 
Gene Name
 MAP3K14 
Gene Synonyms/Alias
 NIK 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20GSAVGQQKELPKAKEubiquitination[1]
41KKQSSVYKLEAVEKSubiquitination[1]
47YKLEAVEKSPVFCGKubiquitination[1]
54KSPVFCGKWEILNDVubiquitination[2]
106RIFIAGSKQYSQSESubiquitination[1, 2, 3, 4]
128VAHATEGKMARVCWKubiquitination[3, 4]
163HAGVALAKPLPRTPEubiquitination[1, 3, 4]
198RNTPQFTKPLKEPGLubiquitination[1, 2]
201PQFTKPLKEPGLGQLubiquitination[2]
241QCLNHVWKLHHPQDGubiquitination[1]
276FHPLQPWKPHPLESFubiquitination[1]
294LACVDSQKPLPDPHLubiquitination[3]
385EGVLLTEKLKPVDYEubiquitination[2, 3]
387VLLTEKLKPVDYEYRubiquitination[1]
882IREFHRVKVGDIATGubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non- canonical pathway. Could act in a receptor-selective manner. 
Sequence Annotation
 DOMAIN 400 655 Protein kinase.
 NP_BIND 406 414 ATP (By similarity).
 REGION 401 653 Interaction with ZFP91.
 ACT_SITE 515 515 Proton acceptor (By similarity).
 BINDING 429 429 ATP (By similarity).
 MOD_RES 559 559 Phosphothreonine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 947 AA 
Protein Sequence
MAVMEMACPG APGSAVGQQK ELPKAKEKTP PLGKKQSSVY KLEAVEKSPV FCGKWEILND 60
VITKGTAKEG SEAGPAAISI IAQAECENSQ EFSPTFSERI FIAGSKQYSQ SESLDQIPNN 120
VAHATEGKMA RVCWKGKRRS KARKKRKKKS SKSLAHAGVA LAKPLPRTPE QESCTIPVQE 180
DESPLGAPYV RNTPQFTKPL KEPGLGQLCF KQLGEGLRPA LPRSELHKLI SPLQCLNHVW 240
KLHHPQDGGP LPLPTHPFPY SRLPHPFPFH PLQPWKPHPL ESFLGKLACV DSQKPLPDPH 300
LSKLACVDSP KPLPGPHLEP SCLSRGAHEK FSVEEYLVHA LQGSVSSGQA HSLTSLAKTW 360
AARGSRSREP SPKTEDNEGV LLTEKLKPVD YEYREEVHWA THQLRLGRGS FGEVHRMEDK 420
QTGFQCAVKK VRLEVFRAEE LMACAGLTSP RIVPLYGAVR EGPWVNIFME LLEGGSLGQL 480
VKEQGCLPED RALYYLGQAL EGLEYLHSRR ILHGDVKADN VLLSSDGSHA ALCDFGHAVC 540
LQPDGLGKSL LTGDYIPGTE THMAPEVVLG RSCDAKVDVW SSCCMMLHML NGCHPWTQFF 600
RGPLCLKIAS EPPPVREIPP SCAPLTAQAI QEGLRKEPIH RVSAAELGGK VNRALQQVGG 660
LKSPWRGEYK EPRHPPPNQA NYHQTLHAQP RELSPRAPGP RPAEETTGRA PKLQPPLPPE 720
PPEPNKSPPL TLSKEESGMW EPLPLSSLEP APARNPSSPE RKATVPEQEL QQLEIELFLN 780
SLSQPFSLEE QEQILSCLSI DSLSLSDDSE KNPSKASQSS RDTLSSGVHS WSSQAEARSS 840
SWNMVLARGR PTDTPSYFNG VKVQIQSLNG EHLHIREFHR VKVGDIATGI SSQIPAAAFS 900
LVTKDGQPVR YDMEVPDSGI DLQCTLAPDG SFAWSWRVKH GQLENRP 947 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004709; F:MAP kinase kinase kinase activity; IEA:EC.
 GO:0004704; F:NF-kappaB-inducing kinase activity; ISS:UniProtKB.
 GO:0000186; P:activation of MAPKK activity; IEA:GOC.
 GO:0071260; P:cellular response to mechanical stimulus; IEP:UniProtKB.
 GO:0007249; P:I-kappaB kinase/NF-kappaB cascade; ISS:UniProtKB.
 GO:0006955; P:immune response; ISS:UniProtKB.
 GO:0000165; P:MAPK cascade; IEA:GOC.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0031295; P:T cell costimulation; TAS:Reactome. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR017425; MAPKKK14.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS