UniProt Accession

 MOBA_ECOLI; P32173; Q2M8F5; Q9LBV0 

Genbank Protein ID

 L19201; U00096; AP009048 

Genbank Nucleotide ID

 AAB02992.1; AAC76855.1; BAE77451.1 

Protein Name

 Molybdenum cofactor guanylyltransferase 

Protein Synonyms/Alias

 MoCo guanylyltransferase; GTP:molybdopterin guanylyltransferase; Mo-MPT guanylyltransferase; Molybdopterin guanylyltransferase; Molybdopterin-guanine dinucleotide biosynthesis protein A; Molybdopterin-guanine dinucleotide synthase; MGD synthase; Protein FA 

Gene Name

 mobA 

Gene Synonyms/Alias

 chlB; mob; narB; b3857; JW3829 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
25	RRMGGVDKGLLELNG	acetylation	[1]
175	AVDFSDHKDAFVNVN	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo- MPT) cofactor (Moco or molybdenum cofactor) to form Mo- molybdopterin guanine dinucleotide (Mo-MGD) cofactor. Is also involved in the biosynthesis of the bis-MGD form of the Moco cofactor (Mo-bisMGD) in which the metal is symmetrically ligated by the dithiolene groups of two MGD molecules. Is necessary and sufficient for the in vitro activation of the DMSOR molybdoenzyme that uses the Mo-bisMGD form of molybdenum cofactor, which implies formation and efficient insertion of the cofactor into the enzyme without the need of a chaperone. Is specific for GTP since other nucleotides such as ATP and GMP can not be utilized. 

Sequence Annotation

 NP_BIND 12 14 GTP.
 METAL 101 101 Magnesium.
 BINDING 25 25 GTP.
 BINDING 53 53 GTP.
 BINDING 71 71 GTP.
 BINDING 101 101 GTP.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; GTP-binding; Magnesium; Manganese; Metal-binding; Molybdenum cofactor biosynthesis; Nucleotide-binding; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 194 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:HAMAP.
 GO:0070568; F:guanylyltransferase activity; IEA:HAMAP.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006777; P:Mo-molybdopterin cofactor biosynthetic process; IEA:UniProtKB-KW. 

Interpro

 IPR025877; MobA-like_NTP_Trfase_dom.
 IPR013482; Molybde_CF_guanTrfase. 

Pfam

 PF12804; NTP_transf_3 

SMART

  

PROSITE

  

PRINTS