CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015503
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NF-X1-type zinc finger protein NFXL1 
Protein Synonyms/Alias
 Ovarian zinc finger protein; hOZFP 
Gene Name
 NFXL1 
Gene Synonyms/Alias
 OZFP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
209VTDDDFGKKDCPWPCubiquitination[1, 2]
293TTTCYCKKAKPIPRRubiquitination[3]
484QKHQCRRKCCPGNCPubiquitination[2]
527CPETVDVKCNCGNTKubiquitination[4]
534KCNCGNTKVTVPCGRubiquitination[4]
588PCQKVLEKCGHLCPAubiquitination[4]
693DGCTGKNKAGPECLHubiquitination[4]
750SLYVECRKITTADVNubiquitination[3]
759TTADVNEKNLLSCCKubiquitination[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
 ZN_FING 160 220 RING-type; atypical.
 ZN_FING 269 287 NF-X1-type 1.
 ZN_FING 322 341 NF-X1-type 2.
 ZN_FING 375 394 NF-X1-type 3.
 ZN_FING 428 447 NF-X1-type 4.
 ZN_FING 455 476 NF-X1-type 5.
 ZN_FING 480 499 NF-X1-type 6.
 ZN_FING 507 526 NF-X1-type 7.
 ZN_FING 674 702 NF-X1-type 8.
 ZN_FING 715 733 NF-X1-type 9.
 ZN_FING 777 796 NF-X1-type 10.
 MOD_RES 50 50 Phosphoserine.
 MOD_RES 65 65 Phosphoserine.
 MOD_RES 106 106 Phosphoserine.
 MOD_RES 107 107 Phosphoserine.
 MOD_RES 108 108 Phosphoserine.
 MOD_RES 109 109 Phosphoserine.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Membrane; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 911 AA 
Protein Sequence
MEASWRQVAG GRGRSRGRAT AAPSGNGVHL RGAGGGREKG SVGAVPSGTS PGGVATTAAA 60
GSRHSPAGSQ ALQTTAASEL MSQKKFEEIK KANQAAARKL VEEQFSSSSE EGDEDFEGKQ 120
GKILANTFIT YTTQTDGDTR ELERTKQYVN EAFQAGAMTC LICIASVKRN QAVWSCSGCF 180
CIFHMPCIQK WAKDSQFLVS SVTDDDFGKK DCPWPCPKCR FEYKRSETPS RYYCYCGKVE 240
DPPLDPWLVP HSCGQVCERE FKPPCGHKCL LLCHPGPCPP CPKMVTTTCY CKKAKPIPRR 300
CSAKEWSCQL PCGQKLLCGQ HKCENPCHAG SCQPCPRVSR QKCVCGKKVA ERSCASPLWH 360
CDQVCGKTLP CGNHTCEQVC HVGACGECPR SGKRFCPCQK SKFSLPCTED VPTCGDSCDK 420
VLECGIHRCS QRCHRGPCET CRQEVEKHCR CGKHTKRMPC HKPYLCETKC VKMRDCQKHQ 480
CRRKCCPGNC PPCDQNCGRT LGCRNHKCPS VCHRGSCYPC PETVDVKCNC GNTKVTVPCG 540
RERTTRPPKC KEQCSRPPTC HHTSQEKHRC HFGSCPPCHQ PCQKVLEKCG HLCPAPCHDQ 600
ALIKQTGRHQ PTGPWEQPSE PAFIQTALPC PPCQVPIPME CLGKHEVSPL PCHAVGPYSC 660
KRVCGRILDC QNHTCMKECH KVTKTDGCTG KNKAGPECLH CEEGCSKSRP LGCLHPCILR 720
CHPGECPPCV QMLRIKCHCK ITSLYVECRK ITTADVNEKN LLSCCKNQCP KELPCGHRCK 780
EMCHPGECPF NCNQKVKLRC PCKRIKKELQ CNKVRENQVS IECDTTCKEM KRKASEIKEA 840
EAKAALEEEK RRQQAELEAF ENRLKGRRKK NRKRDEVAVE LSLWQKHKYY LISVCGVVVV 900
VFAWYITHDV N 911 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR019786; Zinc_finger_PHD-type_CS.
 IPR000967; Znf_NFX1.
 IPR001841; Znf_RING. 
Pfam
 PF01422; zf-NF-X1 
SMART
 SM00438; ZnF_NFX 
PROSITE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS