CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017008
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 75 
Protein Synonyms/Alias
  
Gene Name
 WDR75 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
123FVIVNKEKPDIFQLVubiquitination[1, 2, 3, 4, 5, 6]
136LVSVKLPKSSSQEVEubiquitination[2, 4, 6]
145SSQEVEAKELSFVLDubiquitination[2]
203SRNKKHAKNNFTCVAubiquitination[4]
226ASGHMDGKIRLWRNFacetylation[6]
226ASGHMDGKIRLWRNFubiquitination[4]
281EWRDATEKNKEFLPRubiquitination[4]
283RDATEKNKEFLPRLGubiquitination[2, 4, 6]
313CTSHSDNKIIIIHRNubiquitination[4]
332AVIQGLVKDRSIFTGubiquitination[2, 4, 6, 7]
347LMIDPRTKALVLNGKubiquitination[4]
354KALVLNGKPGHLQFYubiquitination[1, 4, 5]
411TVEQRQEKETELELQubiquitination[4]
427KLWMYNKKTQGFILNubiquitination[4]
455LCFCNAEKSEQPTLVubiquitination[2]
466PTLVTASKDGYFKVWacetylation[6, 8, 9, 10]
466PTLVTASKDGYFKVWubiquitination[1, 2, 3, 4, 5, 6, 7]
483TDDSDIYKKAVGWTCubiquitination[2, 7]
611GSDLFVFKPSEPRPLubiquitination[2, 4]
628QKGISREKVQWGVFVubiquitination[2, 4]
671SLLTFSTKSPEEKLTubiquitination[2, 4, 6]
676STKSPEEKLTPTSKQubiquitination[2, 4]
682EKLTPTSKQLLAEESubiquitination[2, 4, 7]
701PFYFILGKHRQQQDEubiquitination[1, 4, 5, 6, 7]
810DIIHQLSKSEEKELRubiquitination[2]
821KELRKFRKIDYSWIAubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [10] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
 REPEAT 14 52 WD 1.
 REPEAT 55 95 WD 2.
 REPEAT 201 240 WD 3.
 REPEAT 246 285 WD 4.
 REPEAT 288 328 WD 5.
 REPEAT 332 371 WD 6.
 REPEAT 440 483 WD 7.
 REPEAT 496 534 WD 8.
 REPEAT 537 578 WD 9.
 MOD_RES 466 466 N6-acetyllysine.
 MOD_RES 779 779 Phosphoserine.
 MOD_RES 782 782 Phosphoserine.
 MOD_RES 796 796 Phosphoserine.
 MOD_RES 811 811 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 830 AA 
Protein Sequence
MVEEENIRVV RCGGSELNFR RAVFSADSKY IFCVSGDFVK VYSTVTEECV HILHGHRNLV 60
TGIQLNPNNH LQLYSCSLDG TIKLWDYIDG ILIKTFIVGC KLHALFTLAQ AEDSVFVIVN 120
KEKPDIFQLV SVKLPKSSSQ EVEAKELSFV LDYINQSPKC IAFGNEGVYV AAVREFYLSV 180
YFFKKKTTSR FTLSSSRNKK HAKNNFTCVA CHPTEDCIAS GHMDGKIRLW RNFYDDKKYT 240
YTCLHWHHDM VMDLAFSVTG TSLLSGGRES VLVEWRDATE KNKEFLPRLG ATIEHISVSP 300
AGDLFCTSHS DNKIIIIHRN LEASAVIQGL VKDRSIFTGL MIDPRTKALV LNGKPGHLQF 360
YSLQSDKQLY NLDIIQQEYI NDYGLIQIEL TKAAFGCFGN WLATVEQRQE KETELELQMK 420
LWMYNKKTQG FILNTKINMP HEDCITALCF CNAEKSEQPT LVTASKDGYF KVWILTDDSD 480
IYKKAVGWTC DFVGSYHKYQ ATNCCFSEDG SLLAVSFEEI VTIWDSVTWE LKCTFCQRAG 540
KIRHLCFGRL TCSKYLLGAT ENGILCCWNL LSCALEWNAK LNVRVMEPDP NSENIAAISQ 600
SSVGSDLFVF KPSEPRPLYI QKGISREKVQ WGVFVPRDVP ESFTSEAYQW LNRSQFYFLT 660
KSQSLLTFST KSPEEKLTPT SKQLLAEESL PTTPFYFILG KHRQQQDEKL NETLENELVQ 720
LPLTENIPAI SELLHTPAHV LPSAAFLCSM FVNSLLLSKE TKSAKEIPED VDMEEEKESE 780
DSDEENDFTE KVQDTSNTGL GEDIIHQLSK SEEKELRKFR KIDYSWIAAL 830 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS