UniProt Accession

 DLG4_RAT; P31016; P97631 

Genbank Protein ID

 M96853; X66474; U77090 

Genbank Nucleotide ID

 AAA41971.1; CAA47103.1; AAB38270.1 

Protein Name

 Disks large homolog 4 

Protein Synonyms/Alias

 Postsynaptic density protein 95; PSD-95; Synapse-associated protein 90; SAP-90; SAP90 

Gene Name

 Dlg4 

Gene Synonyms/Alias

 Dlgh4; Psd95 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
138	SAAVEALKEAGSIVR	acetylation	[1]
138	SAAVEALKEAGSIVR	ubiquitination	[2]
157	RRKPPAEKVMEIKLI	ubiquitination	[2]
162	AEKVMEIKLIKGPKG	acetylation	[1]
162	AEKVMEIKLIKGPKG	ubiquitination	[2]
202	IEGGAAHKDGRLQIG	ubiquitination	[2]
441	RALFDYDKTKDCGFL	ubiquitination	[2]
591	SSREKMEKDIQAHKF	ubiquitination	[2]
597	EKDIQAHKFIEAGQY	acetylation	[1]
663	ENVLEINKRITEEQA	acetylation	[1]
663	ENVLEINKRITEEQA	ubiquitination	[2]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. 

Sequence Annotation

 DOMAIN 65 151 PDZ 1.
 DOMAIN 160 246 PDZ 2.
 DOMAIN 313 393 PDZ 3.
 DOMAIN 428 498 SH3.
 DOMAIN 534 709 Guanylate kinase-like.
 MOD_RES 142 142 Phosphoserine (By similarity).
 MOD_RES 240 240 Phosphotyrosine (By similarity).
 MOD_RES 295 295 Phosphoserine (By similarity).
 MOD_RES 397 397 Phosphotyrosine (By similarity).
 MOD_RES 415 415 Phosphoserine (By similarity).
 MOD_RES 418 418 Phosphoserine (By similarity).
 MOD_RES 432 432 Phosphotyrosine (By similarity).
 MOD_RES 580 580 Phosphotyrosine (By similarity).
 MOD_RES 604 604 Phosphotyrosine (By similarity).
 MOD_RES 715 715 Phosphotyrosine (By similarity).
 LIPID 3 3 S-palmitoyl cysteine.
 LIPID 5 5 S-palmitoyl cysteine.  

Keyword

 3D-structure; Alternative splicing; Cell junction; Cell membrane; Complete proteome; Direct protein sequencing; Lipoprotein; Membrane; Palmitate; Phosphoprotein; Postsynaptic cell membrane; Reference proteome; Repeat; SH3 domain; Synapse; Synaptosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 724 AA 

Protein Sequence

Gene Ontology

 GO:0032281; C:alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex; IEA:Compara.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0032839; C:dendrite cytoplasm; IDA:BHF-UCL.
 GO:0060076; C:excitatory synapse; IDA:BHF-UCL.
 GO:0031234; C:extrinsic to internal side of plasma membrane; IEA:Compara.
 GO:0044224; C:juxtaparanode region of axon; IDA:MGI.
 GO:0044306; C:neuron projection terminus; IEA:Compara.
 GO:0014069; C:postsynaptic density; IDA:UniProtKB.
 GO:0045211; C:postsynaptic membrane; IDA:MGI.
 GO:0008021; C:synaptic vesicle; IEA:Compara.
 GO:0033130; F:acetylcholine receptor binding; IDA:RGD.
 GO:0097110; F:scaffold protein binding; ISS:BHF-UCL.
 GO:0097113; P:alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor clustering; IMP:BHF-UCL.
 GO:0060997; P:dendritic spine morphogenesis; IMP:BHF-UCL.
 GO:0045184; P:establishment of protein localization; ISS:BHF-UCL.
 GO:0035641; P:locomotory exploration behavior; ISS:BHF-UCL.
 GO:0002091; P:negative regulation of receptor internalization; IDA:UniProtKB.
 GO:0050885; P:neuromuscular process controlling balance; ISS:BHF-UCL.
 GO:0045161; P:neuronal ion channel clustering; TAS:UniProtKB.
 GO:2000463; P:positive regulation of excitatory postsynaptic membrane potential; IDA:BHF-UCL.
 GO:0050806; P:positive regulation of synaptic transmission; IDA:BHF-UCL.
 GO:0006461; P:protein complex assembly; ISS:BHF-UCL.
 GO:0097120; P:receptor localization to synapse; IMP:BHF-UCL.
 GO:2000821; P:regulation of grooming behavior; ISS:BHF-UCL.
 GO:0048169; P:regulation of long-term neuronal synaptic plasticity; IEA:Compara.
 GO:2000310; P:regulation of N-methyl-D-aspartate selective glutamate receptor activity; IDA:BHF-UCL.
 GO:0035176; P:social behavior; ISS:BHF-UCL.
 GO:0016188; P:synaptic vesicle maturation; IEA:Compara.
 GO:0071625; P:vocalization behavior; ISS:BHF-UCL. 

Interpro

 IPR008144; Guanylate_kin.
 IPR008145; Guanylate_kin/L-typ_Ca_channel.
 IPR020590; Guanylate_kinase_CS.
 IPR016313; M-assoc_guanylate_kinase.
 IPR019590; MAGUK_PEST_N.
 IPR027417; P-loop_NTPase.
 IPR001478; PDZ.
 IPR019583; PDZ_assoc.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 

Pfam

 PF00625; Guanylate_kin
 PF10608; MAGUK_N_PEST
 PF00595; PDZ
 PF10600; PDZ_assoc
 PF07653; SH3_2 

SMART

 SM00072; GuKc
 SM00228; PDZ
 SM00326; SH3 

PROSITE

 PS00856; GUANYLATE_KINASE_1
 PS50052; GUANYLATE_KINASE_2
 PS50106; PDZ
 PS50002; SH3 

PRINTS