UniProt Accession

 FEN1_YEAST; P26793; D6VXH5 

Genbank Protein ID

 S93804; Z28113; BK006944 

Genbank Nucleotide ID

 AAB21998.1; CAA81953.1; DAA09045.1 

Protein Name

 Flap endonuclease 1 

Protein Synonyms/Alias

 FEN-1; Flap structure-specific endonuclease 1; RAD2 homolog nuclease 1; RTH1 nuclease; Structure-specific endonuclease RAD27 

Gene Name

 RAD27 

Gene Synonyms/Alias

 FEN1; RTH1; YKL113C; YKL510 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
24	AIRKSDIKSFFGRKV	acetylation	[1]
81	RMIDNGIKPCYVFDG	ubiquitination	[2]
246	VGPVTALKLIKTHGS	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. 

Sequence Annotation

 REGION 1 105 N-domain.
 REGION 120 251 I-domain.
 REGION 339 347 Interaction with PCNA (By similarity).
 METAL 34 34 Magnesium 1 (By similarity).
 METAL 87 87 Magnesium 1 (By similarity).
 METAL 156 156 Magnesium 1 (By similarity).
 METAL 158 158 Magnesium 1 (By similarity).
 METAL 177 177 Magnesium 2 (By similarity).
 METAL 179 179 Magnesium 2 (By similarity).
 METAL 231 231 Magnesium 2 (By similarity).
 BINDING 47 47 DNA substrate (By similarity).
 BINDING 71 71 DNA substrate (By similarity).
 BINDING 156 156 DNA substrate (By similarity).
 BINDING 229 229 DNA substrate (By similarity).
 BINDING 231 231 DNA substrate (By similarity).  

Keyword

 Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA replication; Endonuclease; Exonuclease; Hydrolase; Magnesium; Metal-binding; Mitochondrion; Nuclease; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 382 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0008409; F:5'-3' exonuclease activity; IDA:SGD.
 GO:0017108; F:5'-flap endonuclease activity; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0006286; P:base-excision repair, base-free sugar-phosphate removal; IMP:SGD.
 GO:0043137; P:DNA replication, removal of RNA primer; IDA:SGD.
 GO:0006303; P:double-strand break repair via nonhomologous end joining; IDA:SGD.
 GO:0000734; P:gene conversion at mating-type locus, DNA repair synthesis; IMP:SGD.
 GO:0001302; P:replicative cell aging; IMP:SGD. 

Interpro

 IPR020045; 5-3_exonuclease_C.
 IPR023426; Flap_endonuc.
 IPR008918; HhH2.
 IPR006086; XPG-I_dom.
 IPR006084; XPG/Rad2.
 IPR019974; XPG_CS.
 IPR006085; XPG_DNA_repair_N. 

Pfam

 PF00867; XPG_I
 PF00752; XPG_N 

SMART

 SM00279; HhH2
 SM00484; XPGI
 SM00485; XPGN 

PROSITE

 PS00841; XPG_1
 PS00842; XPG_2 

PRINTS

 PR00853; XPGRADSUPER.