CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010847
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 N-acetylgalactosamine kinase 
Protein Synonyms/Alias
 GalNAc kinase; Galactokinase 2 
Gene Name
 GALK2 
Gene Synonyms/Alias
 GK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
35SKFGSIPKFYVRAPGubiquitination[1, 2]
168VLGRNLSKVELAEICubiquitination[3]
177ELAEICAKSERYIGTubiquitination[4]
252MECRLAAKLLAKYKSubiquitination[4]
264YKSLQWDKVLRLEEVubiquitination[4]
327TQDVLIFKLYQRAKHubiquitination[4]
347ARVLQFKKICEEAPEubiquitination[4]
416VSMVPADKLPSFLANubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Acts on GalNAc. Also acts as a galactokinase when galactose is present at high concentrations. May be involved in a salvage pathway for the reutilization of free GalNAc derived from the degradation of complex carbohydrates. 
Sequence Annotation
 NP_BIND 139 149 ATP.
 REGION 49 52 Substrate binding.
 REGION 187 190 Substrate binding.
 ACT_SITE 190 190 Proton acceptor (By similarity).  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 458 AA 
Protein Sequence
MATESPATRR VQVAEHPRLL KLKEMFNSKF GSIPKFYVRA PGRVNIIGEH IDYCGYSVLP 60
MAVEQDVLIA VEPVKTYALQ LANTNPLYPD FSTSANNIQI DKTKPLWHNY FLCGLKGIQE 120
HFGLSNLTGM NCLVDGNIPP SSGLSSSSAL VCCAGLVTLT VLGRNLSKVE LAEICAKSER 180
YIGTEGGGMD QSISFLAEEG TAKLIEFSPL RATDVKLPSG AVFVIANSCV EMNKAATSHF 240
NIRVMECRLA AKLLAKYKSL QWDKVLRLEE VQAKLGISLE EMLLVTEDAL HPEPYNPEEI 300
CRCLGISLEE LRTQILSPNT QDVLIFKLYQ RAKHVYSEAA RVLQFKKICE EAPENMVQLL 360
GELMNQSHMS CRDMYECSCP ELDQLVDICR KFGAQGSRLT GAGWGGCTVS MVPADKLPSF 420
LANVHKAYYQ RSDGSLAPEK QSLFATKPGG GALVLLEA 458 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004335; F:galactokinase activity; IDA:UniProtKB.
 GO:0033858; F:N-acetylgalactosamine kinase activity; IEA:EC.
 GO:0006012; P:galactose metabolic process; IEA:InterPro. 
Interpro
 IPR000705; Galactokinase.
 IPR019741; Galactokinase_CS.
 IPR019539; GalKase_gal-bd.
 IPR013750; GHMP_kinase_C_dom.
 IPR006204; GHMP_kinase_N_dom.
 IPR006203; GHMP_knse_ATP-bd_CS.
 IPR006206; Mevalonate/galactokinase.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR014721; Ribosomal_S5_D2-typ_fold_subgr. 
Pfam
 PF10509; GalKase_gal_bdg
 PF08544; GHMP_kinases_C
 PF00288; GHMP_kinases_N 
SMART
  
PROSITE
 PS00106; GALACTOKINASE
 PS00627; GHMP_KINASES_ATP 
PRINTS
 PR00473; GALCTOKINASE.
 PR00959; MEVGALKINASE.