CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004351
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NAD(P)H dehydrogenase [quinone] 1 
Protein Synonyms/Alias
 Azoreductase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1; QR1 
Gene Name
 NQO1 
Gene Synonyms/Alias
 DIA4; NMOR1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
23TSFNYAMKEAAAAALubiquitination[1, 2]
31EAAAAALKKKGWEVVubiquitination[1, 2]
33AAAALKKKGWEVVESubiquitination[1, 2]
54FNPIISRKDITGKLKubiquitination[1, 2]
59SRKDITGKLKDPANFacetylation[3]
59SRKDITGKLKDPANFubiquitination[1, 2]
61KDITGKLKDPANFQYubiquitination[1, 2, 4, 5]
77AESVLAYKEGHLSPDubiquitination[1, 2]
90PDIVAEQKKLEAADLubiquitination[1, 2]
91DIVAEQKKLEAADLVubiquitination[1]
209IQILEGWKKRLENIWacetylation[3]
209IQILEGWKKRLENIWubiquitination[1, 2, 5]
210QILEGWKKRLENIWDubiquitination[1, 5]
241QAGFLMKKEVQDEEKubiquitination[1]
248KEVQDEEKNKKFGLSubiquitination[1]
251QDEEKNKKFGLSVGHubiquitination[1, 2, 4]
262SVGHHLGKSIPTDNQacetylation[3]
262SVGHHLGKSIPTDNQubiquitination[1, 2]
271IPTDNQIKARK****ubiquitination[1, 2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. 
Sequence Annotation
 NP_BIND 18 19 FAD.
 NP_BIND 104 107 FAD.
 NP_BIND 148 151 FAD.
 REGION 126 128 Substrate binding.
 BINDING 12 12 FAD.
 BINDING 67 67 FAD.
 BINDING 156 156 FAD.
 BINDING 201 201 FAD.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; FAD; Flavoprotein; NAD; NADP; Oxidoreductase; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 274 AA 
Protein Sequence
MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL 60
KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV 120
FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC 180
GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK 240
KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK 274 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; TAS:ProtInc.
 GO:0003955; F:NAD(P)H dehydrogenase (quinone) activity; TAS:ProtInc.
 GO:0004784; F:superoxide dismutase activity; IEA:Compara.
 GO:0007568; P:aging; IEA:Compara.
 GO:0043086; P:negative regulation of catalytic activity; IEA:Compara.
 GO:0006809; P:nitric oxide biosynthetic process; TAS:ProtInc.
 GO:0043525; P:positive regulation of neuron apoptotic process; IEA:Compara.
 GO:0006521; P:regulation of cellular amino acid metabolic process; TAS:Reactome.
 GO:0032355; P:response to estradiol stimulus; IEA:Compara.
 GO:0045471; P:response to ethanol; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara.
 GO:0006979; P:response to oxidative stress; IEA:Compara.
 GO:0009636; P:response to toxic substance; TAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006801; P:superoxide metabolic process; IEA:Compara.
 GO:0007271; P:synaptic transmission, cholinergic; TAS:ProtInc.
 GO:0006805; P:xenobiotic metabolic process; TAS:ProtInc. 
Interpro
 IPR003680; Flavodoxin_fold. 
Pfam
 PF02525; Flavodoxin_2 
SMART
  
PROSITE
  
PRINTS