CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016157
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Stearoyl-CoA desaturase 5 
Protein Synonyms/Alias
 Acyl-CoA-desaturase 4; HSCD5; Stearoyl-CoA 9-desaturase 
Gene Name
 SCD5 
Gene Synonyms/Alias
 ACOD4; SCD4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10GPATDAGKIPFCDAKubiquitination[1]
17KIPFCDAKEEIRAGLubiquitination[1, 2]
136RDHRAHHKYSETDADubiquitination[1, 3, 4]
173DVIEKGRKLDVTDLLubiquitination[1, 2, 5]
315TDRKRATKPMIEARKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Fatty acid delta-9-desaturase that introduces a double bond in fatty acyl-coenzyme A at the delta-9 position. 
Sequence Annotation
 MOTIF 94 99 Histidine box-1 (By similarity).
 MOTIF 131 135 Histidine box-2 (By similarity).
 MOTIF 272 276 Histidine box-3 (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism; Iron; Lipid biosynthesis; Lipid metabolism; Membrane; Metal-binding; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 330 AA 
Protein Sequence
MPGPATDAGK IPFCDAKEEI RAGLESSEGG GGPERPGARG QRQNIVWRNV VLMSLLHLGA 60
VYSLVLIPKA KPLTLLWAYF CFLLAALGVT AGAHRLWSHR SYRAKLPLRI FLAVANSMAF 120
QNDIFEWSRD HRAHHKYSET DADPHNARRG FFFSHIGWLF VRKHRDVIEK GRKLDVTDLL 180
ADPVVRIQRK YYKISVVLMC FVVPTLVPWY IWGESLWNSY FLASILRYTI SLNISWLVNS 240
AAHMYGNRPY DKHISPRQNP LVALGAIGEG FHNYHHTFPF DYSASEFGLN FNPTTWFIDF 300
MCWLGLATDR KRATKPMIEA RKARTGDSSA 330 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0004768; F:stearoyl-CoA 9-desaturase activity; IEA:EC.
 GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW. 
Interpro
 IPR005804; Fatty_acid_desaturase-1.
 IPR001522; Fatty_acid_desaturase-1_C.
 IPR015876; Fatty_acid_desaturase-1_core. 
Pfam
 PF00487; FA_desaturase 
SMART
  
PROSITE
 PS00476; FATTY_ACID_DESATUR_1 
PRINTS
 PR00075; FACDDSATRASE.