CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010821
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleolysin TIAR 
Protein Synonyms/Alias
 TIA-1-related protein 
Gene Name
 TIAL1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
122SAFAPFGKISDARVVacetylation[1]
122SAFAPFGKISDARVVubiquitination[2, 3]
136VKDMATGKSKGYGFVubiquitination[3]
189STQENNTKQLRFEDVubiquitination[3, 4]
203VVNQSSPKNCTVYCGubiquitination[5]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 RNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. 
Sequence Annotation
 DOMAIN 9 85 RRM 1.
 DOMAIN 97 175 RRM 2.
 DOMAIN 205 277 RRM 3.
 MOD_RES 122 122 N6-acetyllysine.
 MOD_RES 201 201 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 375 AA 
Protein Sequence
MMEDDGQPRT LYVGNLSRDV TEVLILQLFS QIGPCKSCKM ITEHTSNDPY CFVEFYEHRD 60
AAAALAAMNG RKILGKEVKV NWATTPSSQK KDTSNHFHVF VGDLSPEITT EDIKSAFAPF 120
GKISDARVVK DMATGKSKGY GFVSFYNKLD AENAIVHMGG QWLGGRQIRT NWATRKPPAP 180
KSTQENNTKQ LRFEDVVNQS SPKNCTVYCG GIASGLTDQL MRQTFSPFGQ IMEIRVFPEK 240
GYSFVRFSTH ESAAHAIVSV NGTTIEGHVV KCYWGKESPD MTKNFQQVDY SQWGQWSQVY 300
GNPQQYGQYM ANGWQVPPYG VYGQPWNQQG FGVDQSPSAA WMGGFGAQPP QGQAPPPVIP 360
PPNQAGYGMA SYQTQ 375 
Gene Ontology
 GO:0010494; C:cytoplasmic stress granule; ISS:UniProtKB.
 GO:0005764; C:lysosome; TAS:ProtInc.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0017091; F:AU-rich element binding; IEA:Compara.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0006952; P:defense response; TAS:ProtInc.
 GO:0007281; P:germ cell development; IEA:Compara.
 GO:0006917; P:induction of apoptosis; TAS:ProtInc.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0017145; P:stem cell division; IEA:Compara. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
 PF00076; RRM_1 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS