CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003844
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Thyroid hormone receptor alpha 
Protein Synonyms/Alias
 Nuclear receptor subfamily 1 group A member 1; V-erbA-related protein 7; EAR-7; c-erbA-1; c-erbA-alpha 
Gene Name
 THRA 
Gene Synonyms/Alias
 EAR7; ERBA1; NR1A1; THRA1; THRA2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
130DLVLDDSKRVAKRKLacetylation[1]
134DDSKRVAKRKLIEQNacetylation[1]
136SKRVAKRKLIEQNREacetylation[1]
190SHWKQRRKFLPDDIGubiquitination[2, 3]
Reference
 [1] Residues K128, 132, and 134 in the thyroid hormone receptor-alpha are essential for receptor acetylation and activity.
 Sánchez-Pacheco A, Martínez-Iglesias O, Méndez-Pertuz M, Aranda A.
 Endocrinology. 2009 Nov;150(11):5143-52. [PMID: 19819978]
 [2] Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells.
 Tan F, Lu L, Cai Y, Wang J, Xie Y, Wang L, Gong Y, Xu BE, Wu J, Luo Y, Qiang B, Yuan J, Sun X, Peng X.
 Proteomics. 2008 Jul;8(14):2885-96. [PMID: 18655026]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. 
Sequence Annotation
 DNA_BIND 53 127 Nuclear receptor.
 ZN_FING 53 73 NR C4-type.
 ZN_FING 91 115 NR C4-type.
 REGION 1 52 Modulating.
 REGION 190 370 Ligand-binding.
 BINDING 228 228 Thyroid hormone.
 BINDING 277 277 Thyroid hormone; via amide nitrogen.
 CROSSLNK 190 190 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Congenital hypothyroidism; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Receptor; Reference proteome; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 490 AA 
Protein Sequence
MEQKPSKVEC GSDPEENSAR SPDGKRKRKN GQCSLKTSMS GYIPSYLDKD EQCVVCGDKA 60
TGYHYRCITC EGCKGFFRRT IQKNLHPTYS CKYDSCCVID KITRNQCQLC RFKKCIAVGM 120
AMDLVLDDSK RVAKRKLIEQ NRERRRKEEM IRSLQQRPEP TPEEWDLIHI ATEAHRSTNA 180
QGSHWKQRRK FLPDDIGQSP IVSMPDGDKV DLEAFSEFTK IITPAITRVV DFAKKLPMFS 240
ELPCEDQIIL LKGCCMEIMS LRAAVRYDPE SDTLTLSGEM AVKREQLKNG GLGVVSDAIF 300
ELGKSLSAFN LDDTEVALLQ AVLLMSTDRS GLLCVDKIEK SQEAYLLAFE HYVNHRKHNI 360
PHFWPKLLMK EREVQSSILY KGAAAEGRPG GSLGVHPEGQ QLLGMHVVQG PQVRQLEQQL 420
GEAGSLQGPV LQHQSPKSPQ QRLLELLHRS GILHARAVCG EDDSSEADSP SSSEEEPEVC 480
EDLAGNAASP 490 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0031490; F:chromatin DNA binding; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003707; F:steroid hormone receptor activity; IEA:InterPro.
 GO:0002153; F:steroid receptor RNA activator RNA binding; IEA:Compara.
 GO:0017025; F:TBP-class protein binding; IDA:UniProtKB.
 GO:0070324; F:thyroid hormone binding; IDA:UniProtKB.
 GO:0004887; F:thyroid hormone receptor activity; IDA:UniProtKB.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:BHF-UCL.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0001502; P:cartilage condensation; IEA:Compara.
 GO:0042994; P:cytoplasmic sequestering of transcription factor; IEA:Compara.
 GO:0030218; P:erythrocyte differentiation; IEA:Compara.
 GO:0008050; P:female courtship behavior; IEA:Compara.
 GO:0009755; P:hormone-mediated signaling pathway; IDA:BHF-UCL.
 GO:0007611; P:learning or memory; IEA:Compara.
 GO:2000143; P:negative regulation of DNA-dependent transcription, initiation; IDA:UniProtKB.
 GO:0017055; P:negative regulation of RNA polymerase II transcriptional preinitiation complex assembly; IDA:UniProtKB.
 GO:0001503; P:ossification; IEA:Compara.
 GO:0045925; P:positive regulation of female receptivity; IEA:Compara.
 GO:0010831; P:positive regulation of myotube differentiation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0008016; P:regulation of heart contraction; IEA:Compara.
 GO:0050994; P:regulation of lipid catabolic process; IEA:Compara.
 GO:0033032; P:regulation of myeloid cell apoptotic process; IEA:Compara.
 GO:0002155; P:regulation of thyroid hormone mediated signaling pathway; IEA:Compara.
 GO:0009409; P:response to cold; IEA:Compara.
 GO:0030878; P:thyroid gland development; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome. 
Interpro
 IPR008946; Nucl_hormone_rcpt_ligand-bd.
 IPR000536; Nucl_hrmn_rcpt_lig-bd_core.
 IPR001723; Str_hrmn_rcpt.
 IPR001728; ThyrH_rcpt.
 IPR001628; Znf_hrmn_rcpt.
 IPR013088; Znf_NHR/GATA. 
Pfam
 PF00104; Hormone_recep
 PF00105; zf-C4 
SMART
 SM00430; HOLI
 SM00399; ZnF_C4 
PROSITE
 PS00031; NUCLEAR_REC_DBD_1
 PS51030; NUCLEAR_REC_DBD_2 
PRINTS
 PR00398; STRDHORMONER.
 PR00047; STROIDFINGER.
 PR00546; THYROIDHORMR.