UniProt Accession

 NIT2_HUMAN; Q9NQR4; B2R9A3; D3DN47; Q8WUF0 

Genbank Protein ID

 AF284574; AF260334; AK313704; CH471052; CH471052; BC020620; BC107890 

Genbank Nucleotide ID

 AAF87103.1; AAG44665.1; BAG36450.1; EAW79824.1; EAW79825.1; AAH20620.1; AAI07891.1 

Protein Name

 Omega-amidase NIT2 

Protein Synonyms/Alias

 Nitrilase homolog 2 

Gene Name

 NIT2 

Gene Synonyms/Alias

 CUA002 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
37	EAATQGAKIVSLPEC	ubiquitination	[1]
52	FNSPYGAKYFPEYAE	ubiquitination	[1]
60	YFPEYAEKIPGESTQ	ubiquitination	[1]
112	TLLAKYRKIHLFDID	ubiquitination	[1]
130	KITFQESKTLSPGDS	ubiquitination	[1]
249	VYSDIDLKKLAEIRQ	ubiquitination	[1]
250	YSDIDLKKLAEIRQQ	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha- ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle. 

Sequence Annotation

 DOMAIN 4 270 CN hydrolase.
 ACT_SITE 43 43 Proton acceptor (Potential).
 ACT_SITE 112 112 Potential.
 ACT_SITE 153 153 Nucleophile (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Hydrolase; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 276 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0050152; F:omega-amidase activity; IEA:EC.
 GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro. 

Interpro

 IPR003010; C-N_Hydrolase. 

Pfam

 PF00795; CN_hydrolase 

SMART

  

PROSITE

 PS50263; CN_HYDROLASE
 PS01227; UPF0012 

PRINTS