CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014092
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase MRCK alpha 
Protein Synonyms/Alias
 CDC42-binding protein kinase alpha; DMPK-like alpha; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; MRCK alpha; Myotonic dystrophy protein kinase-like alpha 
Gene Name
 CDC42BPA 
Gene Synonyms/Alias
 KIAA0451 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
696LKTDLEKKSIFYEEEubiquitination[1]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake. 
Sequence Annotation
 DOMAIN 77 343 Protein kinase.
 DOMAIN 344 414 AGC-kinase C-terminal.
 DOMAIN 1082 1201 PH.
 DOMAIN 1227 1499 CNH.
 DOMAIN 1571 1584 CRIB.
 NP_BIND 83 91 ATP (By similarity).
 ZN_FING 1012 1062 Phorbol-ester/DAG-type.
 ACT_SITE 201 201 Proton acceptor (By similarity).
 BINDING 106 106 ATP.
 MOD_RES 222 222 Phosphoserine; by autocatalysis.
 MOD_RES 234 234 Phosphoserine; by autocatalysis.
 MOD_RES 240 240 Phosphothreonine; by autocatalysis.
 MOD_RES 1127 1127 Phosphoserine (By similarity).
 MOD_RES 1545 1545 Phosphoserine.
 MOD_RES 1651 1651 Phosphoserine.
 MOD_RES 1719 1719 Phosphoserine.
 MOD_RES 1721 1721 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1732 AA 
Protein Sequence
MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE KNILEYLEWA 60
KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD KVFAMKILNK WEMLKRAETA 120
CFREERDVLV NGDNKWITTL HYAFQDDNNL YLVMDYYVGG DLLTLLSKFE DRLPEDMARF 180
YLAEMVIAID SVHQLHYVHR DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT 240
PDYISPEILQ AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER 300
FQFPAQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN IRNCEAPYIP 360
EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV GFTYTSSCVL SDRSCLRVTA 420
GPTSLDLDVN VQRTLDNNLA TEAYERRIKR LEQEKLELSR KLQESTQTVQ ALQYSTVDGP 480
LTASKDLEIK NLKEEIEKLR KQVTESSHLE QQLEEANAVR QELDDAFRQI KAYEKQIKTL 540
QQEREDLNKE LVQASERLKN QSKELKDAHC QRKLAMQEFM EINERLTELH TQKQKLARHV 600
RDKEEEVDLV MQKVESLRQE LRRTERAKKE LEVHTEALAA EASKDRKLRE QSEHYSKQLE 660
NELEGLKQKQ ISYSPGVCSI EHQQEITKLK TDLEKKSIFY EEELSKREGI HANEIKNLKK 720
ELHDSEGQQL ALNKEIMILK DKLEKTRRES QSEREEFESE FKQQYEREKV LLTEENKKLT 780
SELDKLTTLY ENLSIHNQQL EEEVKDLADK KESVAHWEAQ ITEIIQWVSD EKDARGYLQA 840
LASKMTEELE ALRNSSLGTR ATDMPWKMRR FAKLDMSARL ELQSALDAEI RAKQAIQEEL 900
NKVKASNIIT ECKLKDSEKK NLELLSEIEQ LIKDTEELRS EKGIEHQDSQ HSFLAFLNTP 960
TDALDQFETV DSTPLSVHTP TLRKKGCPGS TGFPPKRKTH QFFVKSFTTP TKCHQCTSLM 1020
VGLIRQGCSC EVCGFSCHIT CVNKAPTTCP VPPEQTKGPL GIDPQKGIGT AYEGHVRIPK 1080
PAGVKKGWQR ALAIVCDFKL FLYDIAEGKA SQPSVVISQV IDMRDEEFSV SSVLASDVIH 1140
ASRKDIPCIF RVTASQLSAS NNKCSILMLA DTENEKNKWV GVLSELHKIL KKNKFRDRSV 1200
YVPKEAYDST LPLIKTTQAA AIIDHERIAL GNEEGLFVVH VTKDEIIRVG DNKKIHQIEL 1260
IPNDQLVAVI SGRNRHVRLF PMSALDGRET DFYKLSETKG CQTVTSGKVR HGALTCLCVA 1320
MKRQVLCYEL FQSKTRHRKF KEIQVPYNVQ WMAIFSEQLC VGFQSGFLRY PLNGEGNPYS 1380
MLHSNDHTLS FIAHQPMDAI CAVEISSKEY LLCFNSIGIY TDCQGRRSRQ QELMWPANPS 1440
SCCYNAPYLS VYSENAVDIF DVNSMEWIQT LPLKKVRPLN NEGSLNLLGL ETIRLIYFKN 1500
KMAEGDELVV PETSDNSRKQ MVRNINNKRR YSFRVPEEER MQQRREMLRD PEMRNKLISN 1560
PTNFNHIAHM GPGDGIQILK DLPMPGFPYP SPHHHSGLIS SPINFEHIYH MTVNSAEKFL 1620
SPDSINPEYS PSLRSVPGTP SFMTLRNPRP QESRTVFSGS VSIPSITKSR PEPGRSMSAS 1680
SGLSARSSAQ NGSALKREFS GGSYSAKRQP MPSPSEGSLS SGGMDQGSDA PARDFDGEDS 1740
DSPRHSTASN SSNLSSPPSP ASPRKTKSLS LESTDRGSWD P 1781 
Gene Ontology
 GO:0042641; C:actomyosin; IDA:UniProtKB.
 GO:0031252; C:cell leading edge; ISS:UniProtKB.
 GO:0005911; C:cell-cell junction; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0005083; F:small GTPase regulator activity; IEA:InterPro.
 GO:0031532; P:actin cytoskeleton reorganization; IDA:UniProtKB.
 GO:0031032; P:actomyosin structure organization; IMP:UniProtKB.
 GO:0016477; P:cell migration; IMP:UniProtKB.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0000226; P:microtubule cytoskeleton organization; IEA:Compara.
 GO:0007097; P:nuclear migration; IEA:Compara. 
Interpro
 IPR000961; AGC-kinase_C.
 IPR001180; Citron.
 IPR011009; Kinase-like_dom.
 IPR014930; Myotonic_dystrophy_kinase_coil.
 IPR000095; PAK_box_Rho-bd.
 IPR017892; Pkinase_C.
 IPR001849; Pleckstrin_homology.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS.
 IPR026611; Ser/Thr_kinase_MRCK. 
Pfam
 PF00130; C1_1
 PF00780; CNH
 PF08826; DMPK_coil
 PF00069; Pkinase
 PF00433; Pkinase_C 
SMART
 SM00109; C1
 SM00036; CNH
 SM00285; PBD
 SM00233; PH
 SM00133; S_TK_X
 SM00220; S_TKc 
PROSITE
 PS51285; AGC_KINASE_CTER
 PS50219; CNH
 PS50108; CRIB
 PS50003; PH_DOMAIN
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS