CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015724
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cysteine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Cysteinyl-tRNA synthetase; CysRS 
Gene Name
 Aats-cys 
Gene Synonyms/Alias
 CG8431 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
602REQAKTLKAFDILKLacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
  
Sequence Annotation
 MOTIF 48 58 "HIGH" region (By similarity).
 MOTIF 398 402 "KMSKS" region (By similarity).
 METAL 46 46 Zinc (By similarity).
 METAL 340 340 Zinc (By similarity).
 METAL 365 365 Zinc (By similarity).
 METAL 369 369 Zinc (By similarity).
 BINDING 401 401 ATP (By similarity).
 MOD_RES 297 297 Phosphoserine.  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Metal-binding; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 741 AA 
Protein Sequence
MSKRGQPAWQ APEAVDRPKL KLFNSLTRQK EDFVPLDGNN VTWYSCGPTV YDASHMGHAR 60
SYISFDILRR ILSDYFGYNI HYVMNITDID DKIIRRARQN HLFDEYAAEA QKLPLDELLG 120
QQKEVLQRFQ DTCAKNTDPD KKIMLDKTLQ RMNDAVEALT KAVGKGDERE ISEKRLLYLN 180
EAKDPISDWL DSLKGAQIND NAVFEALPRY WEDQFHNDMK SLNILPPDVL TRVSEYVPQI 240
VTFIQKIIDN GLAYAANNSV YFDVNGFDKR EKHHYAKLVP EAYGDTKSLQ EGEGDLSIAE 300
DRLSEKRSAN DFALWKASKA GEPWWDSPWG KGRPGWHIEC SAMASDIFGP TFDIHTGGVD 360
LKFPHHDNEL AQSEAAFNES EWVKYFLHTG HLTIAGCKMS KSLKNFVTIQ EALKKHSATQ 420
LRLAFLLHSW KDTLDYSENT MEMATQYEKF LNEFFLNVKD LTRHVLSEEP RRQFDAWTEV 480
EAALQKKFSN AQVQVHASLC DNIDTRSALD AIRELVSVSN VYIRDNKTRL NSLLLRNVAT 540
YITDLLHVFG AISGPRGGIG FPVSGGSGAQ AAGADLETTV LPYVQSLAEF RYLVREQAKT 600
LKAFDILKLC DDLRDNVLPN LGVRLEDKDI GKYAVKLVDR DSLLREREAK LAAEAEKAAE 660
KERKKQAAAE AAAAKEAQRR VNPKEMFLAE TEKYSAFDEN GLPTHDKEGK EVSKGQIKKL 720
QKLQQQQEQR YNEYLASIEK A 741 
Gene Ontology
 GO:0005829; C:cytosol; IBA:RefGenome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004817; F:cysteine-tRNA ligase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000049; F:tRNA binding; ISS:UniProtKB.
 GO:0006423; P:cysteinyl-tRNA aminoacylation; ISS:UniProtKB. 
Interpro
 IPR015803; Cys-tRNA-ligase.
 IPR024909; Cys-tRNA/MSH_ligase.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd. 
Pfam
 PF01406; tRNA-synt_1e 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00983; TRNASYNTHCYS.