CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023682
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 256 
Protein Synonyms/Alias
 Bone marrow zinc finger 3; BMZF-3 
Gene Name
 ZNF256 
Gene Synonyms/Alias
 BMZF3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
175FTCKEVGKDFLVRSRubiquitination[1]
307CGKLFNRKYDLLIHQubiquitination[1]
447CGKLFSRKFDLIVHEubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Transcriptional repressor that plays a role in cell proliferation. Requires TRIM28 for its activity. 
Sequence Annotation
 DOMAIN 14 96 KRAB.
 ZN_FING 90 112 C2H2-type 1.
 ZN_FING 239 261 C2H2-type 2.
 ZN_FING 267 289 C2H2-type 3.
 ZN_FING 295 317 C2H2-type 4.
 ZN_FING 323 345 C2H2-type 5.
 ZN_FING 351 373 C2H2-type 6.
 ZN_FING 379 401 C2H2-type 7.
 ZN_FING 407 429 C2H2-type 8.
 ZN_FING 435 457 C2H2-type 9.
 ZN_FING 463 485 C2H2-type 10.
 ZN_FING 491 513 C2H2-type 11.
 ZN_FING 519 541 C2H2-type 12.
 ZN_FING 547 569 C2H2-type 13.
 ZN_FING 575 597 C2H2-type 14.
 ZN_FING 603 625 C2H2-type 15.  
Keyword
 Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Nucleus; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 627 AA 
Protein Sequence
MAAAELTAPA QGIVTFEDVA VYFSWKEWGL LDEAQKCLYH DVMLENLTLT TSLGGSGAGD 60
EEAPYQQSTS PQRVSQVRIP KALPSPQKTN PCEICGPVLR QILHLVEHQG THHGQKLYTD 120
GACRKQLQFT AYLHQHQKQH VGQKHFRSNG GRDMFLSSCT FEVSGKPFTC KEVGKDFLVR 180
SRFLQQQAAH TRKKSNRTKS AVAFHSVKNH YNWGECVKAF SYKHVRVQHQ GDLIRERSYM 240
CSECGKSFST SCSLSDHLRV HTSEKPYTCG ECGKSYRQSS SLITHRRIHT GVRPHQCDEC 300
GKLFNRKYDL LIHQRVHTGE RPYKCSECGK SFSHSSSLIT HQRIHTGMRP YECSECGKSF 360
IHSSSLITHQ RVHTGTRPYM CSECGKSFSQ SCHLIKHRRL HIGEGPYECS ECGKLFTYRS 420
RFFQHQRVHT GVRSHECHEC GKLFSRKFDL IVHERVHTGE RPYECSECGK SFTCKSYLIS 480
HWKVHTGARP YECGECGKSF THSSTLLQHQ RVHTGERPYE CNECGKFFSQ SSSLIRHRRS 540
HTGERPYECS ECWKSFSNHS SLVKHRRVHT GERPYECSEC GKSFSQSSNL TNHQRIHSGE 600
RPYECSDCGK FFTFNSNLLK HQNVHKG 627 
Gene Ontology
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007275; P:multicellular organismal development; TAS:ProtInc.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001909; Krueppel-associated_box.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF01352; KRAB 
SMART
 SM00349; KRAB
 SM00355; ZnF_C2H2 
PROSITE
 PS50805; KRAB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS