UniProt Accession

 ENT3_YEAST; P47160; D6VWU4 

Genbank Protein ID

 Z49625; AY558084; BK006943 

Genbank Nucleotide ID

 CAA89656.1; AAS56410.1; DAA08910.1 

Protein Name

 Epsin-3 

Protein Synonyms/Alias

  

Gene Name

 ENT3 

Gene Synonyms/Alias

 YJR125C; J2048 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
152	ELLSDDNKIRAERKK	ubiquitination	[1]
184	ADGSLNSKAGFTSTK	ubiquitination	[1, 2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Involved in the recruitment of clathrin to the Golgi network and endosomes to form clathrin coated vesicles. Plays a role in the trafficking of clathrin between the Golgi network and endosomes. Binds to membranes enriched in phosphatidylinositol- 3,5-bisphosphate (PtdIns(3,5)P2) and, in association with VPS27, is involved in protein sorting at the multivesicular body (MVB). 

Sequence Annotation

 DOMAIN 24 157 ENTH.
 MOD_RES 196 196 Phosphoserine.
 MOD_RES 198 198 Phosphoserine.
 MOD_RES 203 203 Phosphoserine.
 MOD_RES 212 212 Phosphoserine.
 MOD_RES 223 223 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Golgi apparatus; Lipid-binding; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 408 AA 

Protein Sequence

Gene Ontology

 GO:0030479; C:actin cortical patch; TAS:SGD.
 GO:0030125; C:clathrin vesicle coat; IDA:SGD.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IDA:SGD.
 GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:SGD.
 GO:0000147; P:actin cortical patch assembly; ISS:SGD.
 GO:0007015; P:actin filament organization; ISS:SGD.
 GO:0034498; P:early endosome to Golgi transport; IGI:SGD.
 GO:0006897; P:endocytosis; ISS:SGD.
 GO:0006895; P:Golgi to endosome transport; IMP:SGD.
 GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR008942; ENTH_VHS.
 IPR013809; Epsin-like_N.
 IPR001026; Epsin_dom_N. 

Pfam

 PF01417; ENTH 

SMART

 SM00273; ENTH 

PROSITE

 PS50942; ENTH 

PRINTS