CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012379
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neutral alpha-glucosidase AB 
Protein Synonyms/Alias
 Alpha-glucosidase 2; Glucosidase II subunit alpha 
Gene Name
 GANAB 
Gene Synonyms/Alias
 G2AN; KIAA0088 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
291HADNLRLKVTEGGEPubiquitination[1]
494AIVDPHIKVDSGYRVubiquitination[2]
921VVIIGAGKPAAVVLQubiquitination[2]
930AAVVLQTKGSPESRLubiquitination[2]
Reference
 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. 
Sequence Annotation
 ACT_SITE 542 542 Nucleophile.
 ACT_SITE 545 545 By similarity.
 ACT_SITE 618 618 Proton donor (By similarity).
 CARBOHYD 97 97 N-linked (GlcNAc...).  
Keyword
 Alternative splicing; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Glycosidase; Golgi apparatus; Hydrolase; Polymorphism; Reference proteome; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 944 AA 
Protein Sequence
MAAVAAVAAR RRRSWASLVL AFLGVCLGIT LAVDRSNFKT CEESSFCKRQ RSIRPGLSPY 60
RALLDSLQLG PDSLTVHLIH EVTKVLLVLE LQGLQKNMTR FRIDELEPRR PRYRVPDVLV 120
ADPPIARLSV SGRDENSVEL TMAEGPYKII LTARPFRLDL LEDRSLLLSV NARGLLEFEH 180
QRAPRVSFSD KVNLTLGSIW DKIKNLFSRQ GSKDPAEGDG AQPEETPRDG DKPEETQGKA 240
EKDEPGAWEE TFKTHSDSKP YGPMSVGLDF SLPGMEHVYG IPEHADNLRL KVTEGGEPYR 300
LYNLDVFQYE LYNPMALYGS VPVLLAHNPH RDLGIFWLNA AETWVDISSN TAGKTLFGKM 360
MDYLQGSGET PQTDVRWMSE TGIIDVFLLL GPSISDVFRQ YASLTGTQAL PPLFSLGYHQ 420
SRWNYRDEAD VLEVDQGFDD HNLPCDVIWL DIEHADGKRY FTWDPSRFPQ PRTMLERLAS 480
KRRKLVAIVD PHIKVDSGYR VHEELRNLGL YVKTRDGSDY EGWCWPGSAG YPDFTNPTMR 540
AWWANMFSYD NYEGSAPNLF VWNDMNEPSV FNGPEVTMLK DAQHYGGWEH RDVHNIYGLY 600
VHMATADGLR QRSGGMERPF VLARAFFAGS QRFGAVWTGD NTAEWDHLKI SIPMCLSLGL 660
VGLSFCGADV GGFFKNPEPE LLVRWYQMGA YQPFFRAHAH LDTGRREPWL LPSQHNDIIR 720
DALGQRYSLL PFWYTLLYQA HREGIPVMRP LWVQYPQDVT TFNIDDQYLL GDALLVHPVS 780
DSGAHGVQVY LPGQGEVWYD IQSYQKHHGP QTLYLPVTLS SIPVFQRGGT IVPRWMRVRR 840
SSECMKDDPI TLFVALSPQG TAQGELFLDD GHTFNYQTRQ EFLLRRFSFS GNTLVSSSAD 900
PEGHFETPIW IERVVIIGAG KPAAVVLQTK GSPESRLSFQ HDPETSVLVL RKPGINVASD 960
WSIHLR 966 
Gene Ontology
 GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
 GO:0017177; C:glucosidase II complex; IEA:Compara.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0033919; F:glucan 1,3-alpha-glucosidase activity; IEA:EC.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0006457; P:protein folding; TAS:Reactome.
 GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome. 
Interpro
 IPR011013; Gal_mutarotase_SF_dom.
 IPR000322; Glyco_hydro_31.
 IPR025887; Glyco_hydro_31_N_dom.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF13802; Gal_mutarotas_2
 PF01055; Glyco_hydro_31 
SMART
  
PROSITE
 PS00129; GLYCOSYL_HYDROL_F31_1
 PS00707; GLYCOSYL_HYDROL_F31_2 
PRINTS