| Tag | Content |
|---|
| CPLM ID | CPLM-019684 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Pre-rRNA-processing protein PNO1 |
Protein Synonyms/Alias | Partner of NOB1; Ribosomal RNA-processing protein 20 |
Gene Name | PNO1 |
Gene Synonyms/Alias | DIM2; RRP20; YOR145C; O3513; YOR3513C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 154 | TDPGALQKGADFIKA | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Required for small ribosomal subunit (SSU) synthesis. Has a role in the processing of early nucleolar and late cytoplasmic pre-RNA species. Recruits DIM1 to nucleolar pre-RNAs. Indirectly required for cleavage at the A2 site of the 20S pre- rRNA, forming 18S rRNA, and at A1 and A2 sites of other pre-rRNAs. |
Sequence Annotation | DOMAIN 195 247 KH.
MOD_RES 47 47 Phosphoserine.
MOD_RES 51 51 Phosphothreonine. |
Keyword | Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Ribosome biogenesis; RNA-binding. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 274 AA |
Protein Sequence | MVAPTALKKA TVTPVSGQDG GSSRIIGINN TESIDEDDDD DVLLDDSDNN TAKEEVEGEE 60
GSRKTHESKT VVVDDQGKPR FTSASKTQGN KIKFESRKIM VPPHRMTPLR NSWTKIYPPL 120
VEHLKLQVRM NLKTKSVELR TNPKFTTDPG ALQKGADFIK AFTLGFDLDD SIALLRLDDL 180
YIETFEVKDV KTLTGDHLSR AIGRIAGKDG KTKFAIENAT RTRIVLADSK IHILGGFTHI 240
RMARESVVSL ILGSPPGKVY GNLRTVASRL KERY 274 |
Gene Ontology | GO:0030686; C:90S preribosome; IDA:SGD. GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0005730; C:nucleolus; IDA:SGD. GO:0003723; F:RNA binding; IEA:UniProtKB-KW. GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD. GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD. GO:0006461; P:protein complex assembly; IMP:SGD. |
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