UniProt Accession

 B3GA3_HUMAN; O94766; Q96I06; Q9UEP0 

Genbank Protein ID

 AB009598; BC007906; BC071961; AJ005865 

Genbank Nucleotide ID

 BAA34537.1; AAH07906.1; AAH71961.1; CAA06742.1 

Protein Name

 Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 

Protein Synonyms/Alias

 Beta-1,3-glucuronyltransferase 3; Glucuronosyltransferase I; GlcAT-I; UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase; GlcUAT-I 

Gene Name

 B3GAT3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
90	TYARLVQKAELVRLS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. 

Sequence Annotation

 NP_BIND 82 84 UDP-glucuronate binding.
 NP_BIND 194 196 UDP-glucuronate binding.
 NP_BIND 308 310 UDP-glucuronate binding.
 REGION 243 252 Interaction with galactose moiety of
 ACT_SITE 281 281 Proton donor/acceptor.
 METAL 196 196 Manganese.
 BINDING 113 113 UDP-glucuronate.
 BINDING 156 156 UDP-glucuronate.
 BINDING 161 161 UDP-glucuronate.
 CARBOHYD 300 300 N-linked (GlcNAc...).
 DISULFID 33 33 Interchain.  

Keyword

 3D-structure; Complete proteome; Disease mutation; Disulfide bond; Glycoprotein; Golgi apparatus; Manganese; Membrane; Metal-binding; Reference proteome; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 335 AA 

Protein Sequence

Gene Ontology

 GO:0005801; C:cis-Golgi network; IDA:UniProtKB.
 GO:0000139; C:Golgi membrane; NAS:UniProtKB.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0015018; F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity; NAS:UniProtKB.
 GO:0030145; F:manganese ion binding; NAS:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0030204; P:chondroitin sulfate metabolic process; TAS:Reactome.
 GO:0050650; P:chondroitin sulfate proteoglycan biosynthetic process; IDA:MGI.
 GO:0050651; P:dermatan sulfate proteoglycan biosynthetic process; IDA:MGI.
 GO:0015012; P:heparan sulfate proteoglycan biosynthetic process; IDA:MGI.
 GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway. 

Interpro

 IPR005027; Glyco_trans_43. 

Pfam

 PF03360; Glyco_transf_43 

SMART

  

PROSITE

  

PRINTS