UniProt Accession

 TFAM_MOUSE; P40630; P97894; P97906; Q543I8; Q9DBM9 

Genbank Protein ID

 U57939; U63858; BC001987; BC083084; AK004857; AK050446; AK167348; AK167777; AK169808; U63712; L07107 

Genbank Nucleotide ID

 AAC52815.1; AAC52816.1; AAH01987.1; AAH83084.1; BAC34258.1; BAE39447.1; BAE39809.1; BAE41382.1; AAB06395.1; AAA02579.1 

Protein Name

 Transcription factor A, mitochondrial 

Protein Synonyms/Alias

 mtTFA; Testis-specific high mobility group protein; TS-HMG 

Gene Name

 Tfam 

Gene Synonyms/Alias

 Hmgts 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
66	FSTEQLPKFKAKHPD	acetylation	[1]
70	QLPKFKAKHPDAKLS	acetylation	[1]
103	KVYEADFKAEWKAYK	acetylation	[1]
107	ADFKAEWKAYKEAVS	acetylation	[1]
115	AYKEAVSKYKEQLTP	acetylation	[1]
117	KEAVSKYKEQLTPSQ	acetylation	[1]
130	SQLMGMEKEARQRRL	acetylation	[1]
204	QAYIQLAKDDRIRYD	acetylation	[1]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 Isoform Mitochondrial binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. Isoform Nuclear may also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis. 

Sequence Annotation

 DNA_BIND 49 117 HMG box 1.
 DNA_BIND 154 218 HMG box 2.
 MOD_RES 54 54 Phosphoserine; by PKA (By similarity).
 MOD_RES 55 55 Phosphoserine; by PKA (By similarity).
 MOD_RES 60 60 Phosphoserine; by PKA (By similarity).
 MOD_RES 159 159 Phosphoserine; by PKA (By similarity).
 MOD_RES 192 192 Phosphoserine (By similarity).  

Keyword

 Activator; Alternative splicing; Complete proteome; DNA-binding; Mitochondrion; Mitochondrion nucleoid; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 243 AA 

Protein Sequence

Gene Ontology

 GO:0042645; C:mitochondrial nucleoid; ISS:UniProtKB.
 GO:0005667; C:transcription factor complex; TAS:MGI.
 GO:0003682; F:chromatin binding; ISS:UniProtKB.
 GO:0008301; F:DNA binding, bending; ISS:UniProtKB.
 GO:0070363; F:mitochondrial light strand promoter sense binding; ISS:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; ISS:UniProtKB.
 GO:0033108; P:mitochondrial respiratory chain complex assembly; IMP:MGI.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0006391; P:transcription initiation from mitochondrial promoter; ISS:UniProtKB. 

Interpro

 IPR009071; HMG_box_dom. 

Pfam

 PF09011; DUF1898
 PF00505; HMG_box 

SMART

 SM00398; HMG 

PROSITE

 PS50118; HMG_BOX_2 

PRINTS