CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005617
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glycolipid 2-alpha-mannosyltransferase 
Protein Synonyms/Alias
 Alpha-1,2-mannosyltransferase 
Gene Name
 KRE2 
Gene Synonyms/Alias
 MNT1; YDR483W; D8035.26 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
66SEENDAKKLEQSALNubiquitination[1]
96ALKAAAEKADAPIDTubiquitination[1]
142KGLLSSIKYVENKINacetylation[2]
211IRADAATKYIYGGSEubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Required for the attachment of the third mannose residue of O-linked saccharides. 
Sequence Annotation
 REGION 31 118 Stem region.
 REGION 119 442 Catalytic.
 ACT_SITE 329 329 Nucleophile (Potential).
 CARBOHYD 197 197 N-linked (GlcNAc...).  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Glycoprotein; Glycosyltransferase; Golgi apparatus; Manganese; Membrane; Reference proteome; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 442 AA 
Protein Sequence
MALFLSKRLL RFTVIAGAVI VLLLTLNSNS RTQQYIPSSI SAAFDFTSGS ISPEQQVISE 60
ENDAKKLEQS ALNSEASEDS EAMDEESKAL KAAAEKADAP IDTKTTMDYI TPSFANKAGK 120
PKACYVTLVR NKELKGLLSS IKYVENKINK KFPYPWVFLN DEPFTEEFKE AVTKAVSSEV 180
KFGILPKEHW SYPEWINQTK AAEIRADAAT KYIYGGSESY RHMCRYQSGF FWRHELLEEY 240
DWYWRVEPDI KLYCDINYDV FKWMQENEKV YGFTVSIHEY EVTIPTLWQT SMDFIKKNPE 300
YLDENNLMSF LSNDNGKTYN LCHFWSNFEI ANLNLWRSPA YREYFDTLDH QGGFFYERWG 360
DAPVHSIAAA LFLPKDKIHY FSDIGYHHPP YDNCPLDKEV YNSNNCECDQ GNDFTFQGYS 420
CGKEYYDAQG LVKPKNWKKF RE 442 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:SGD.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0000026; F:alpha-1,2-mannosyltransferase activity; IDA:SGD.
 GO:0000032; P:cell wall mannoprotein biosynthetic process; IMP:SGD.
 GO:0006491; P:N-glycan processing; IMP:SGD.
 GO:0006493; P:protein O-linked glycosylation; IMP:SGD. 
Interpro
 IPR002685; Glyco_trans_15. 
Pfam
 PF01793; Glyco_transf_15 
SMART
  
PROSITE
  
PRINTS