CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022330
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Testis-expressed sequence 10 protein 
Protein Synonyms/Alias
  
Gene Name
 TEX10 
Gene Synonyms/Alias
 L18; Nbla10363 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
36TPTNFKTKTIHLPEQubiquitination[1]
197ISHQQLSKGLINRDRubiquitination[1, 2, 3, 4, 5]
404SRFPYVLKEITKHKRubiquitination[3]
529LLLKFFSKIYQTEELubiquitination[3]
590RANKELLKSLQATALubiquitination[1, 3, 6]
889ILVQQIIKNITTLKSacetylation[7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861
Functional Description
 Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. 
Sequence Annotation
 REPEAT 99 137 HEAT.
 MOD_RES 29 29 Phosphothreonine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Membrane; Nucleus; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 929 AA 
Protein Sequence
MTKKRKRQHD FQKVKLKVGK KKPKLQNATP TNFKTKTIHL PEQLKEDGTL PTNNRKLNIK 60
DLLSQMHHYN AGVKQSALLG LKDLLSQYPF IIDAHLSNIL SEVTAVFTDK DANVRLAAVQ 120
LLQFLAPKIR AEQISPFFPL VSAHLSSAMT HITEGIQEDS LKVLDILLEQ YPALITGRSS 180
ILLKNFVELI SHQQLSKGLI NRDRSQSWIL SVNPNRRLTS QQWRLKVLVR LSKFLQALAD 240
GSSRLRESEG LQEQKENPHA TSNSIFINWK EHANDQQHIQ VYENGGSQPN VSSQFRLRYL 300
VGGLSGVDEG LSSTENLKGF IEIIIPLLIE CWVEAVPPQL ATPVGNGIER EPLQVMQQVL 360
NIISLLWKLS KQQDETHKLE SWLRKNYLID FKHHFMSRFP YVLKEITKHK RKEPNKSIKH 420
CTVLSNNIDH LLLNLTLSDI MVSLANASTL QKDCSWIEMI RKFVTETLED GSRLNSKQLN 480
RLLGVSWRLM QIQPNREDTE TLIKAVYTLY QQRGLILPVR TLLLKFFSKI YQTEELRSCR 540
FRYRSKVLSR WLAGLPLQLA HLGSRNPELS TQLIDIIHTA AARANKELLK SLQATALRIY 600
DPQEGAVVVL PADSQQRLVQ LVYFLPSLPA DLLSRLSRCC IMGRLSSSLA AMLIGILHMR 660
SSFSGWKYSA KDWLMSDVDY FSFLFSTLTG FSKEELTWLQ SLRGVPHVIQ TQLSPVLLYL 720
TDLDQFLHHW DVTEAVFHSL LVIPARSQNF DILQSAISKH LVGLTVIPDS TAGCVFGVIC 780
KLLDHTCVVS ETLLPFLASC CYSLLYFLLT IEKGEAEHLR KRDKLWGVCV SILALLPRVL 840
RLMLQSLRVN RVGPEELPVV GQLLRLLLQH APLRTHMLTN AILVQQIIKN ITTLKSGSVQ 900
EQWLTDLHYC FNVYITGHPQ GPSALATVY 929 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0071339; C:MLL1 complex; IDA:UniProtKB.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR021133; HEAT_type_2.
 IPR024679; IPI1/TEX10. 
Pfam
 PF12333; Ipi1_N 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS