CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018324
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PEST proteolytic signal-containing nuclear protein 
Protein Synonyms/Alias
 PCNP; PEST-containing nuclear protein 
Gene Name
 PCNP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
29GPEEEAEKPVKTKTVacetylation[1, 2]
64EAADLPTKPTKISKFacetylation[1, 2, 3, 4, 5]
64EAADLPTKPTKISKFubiquitination[2, 6, 7]
67DLPTKPTKISKFGFAacetylation[4]
82IGSQTTKKASAISIKubiquitination[7]
150AGPNSFNKGKHGFSDacetylation[3, 5]
150AGPNSFNKGKHGFSDubiquitination[7]
152PNSFNKGKHGFSDNQacetylation[2, 3, 8, 9]
152PNSFNKGKHGFSDNQubiquitination[7]
167KLWERNIKSHLGNVHubiquitination[7]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [9] Proteome-wide prediction of acetylation substrates.
 Basu A, Rose KL, Zhang J, Beavis RC, Ueberheide B, Garcia BA, Chait B, Zhao Y, Hunt DF, Segal E, Allis CD, Hake SB.
 Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13785-90. [PMID: 19666589
Functional Description
 May be involved in cell cycle regulation. 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 64 64 N6-acetyllysine.
 MOD_RES 87 87 Phosphoserine.
 MOD_RES 119 119 Phosphoserine.
 MOD_RES 139 139 Phosphothreonine.
 MOD_RES 147 147 Phosphoserine.
 MOD_RES 150 150 N6-acetyllysine.
 MOD_RES 152 152 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Cell cycle; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 178 AA 
Protein Sequence
MADGKAGDEK PEKSQRAGAA GGPEEEAEKP VKTKTVSSSN GGESSSRSAE KRSAEEEAAD 60
LPTKPTKISK FGFAIGSQTT KKASAISIKL GSSKPKETVP TLAPKTLSVA AAFNEDEDSE 120
PEEMPPEAKM RMKNIGRDTP TSAGPNSFNK GKHGFSDNQK LWERNIKSHL GNVHDQDN 178 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HGNC.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:HGNC.
 GO:0016567; P:protein ubiquitination; IDA:HGNC. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS