UniProt Accession

 CYBP_MOUSE; Q9CXW3; O70140 

Genbank Protein ID

 U97327; AK013924; BC025948 

Genbank Nucleotide ID

 AAC16757.1; AAH25948.1 

Protein Name

 Calcyclin-binding protein 

Protein Synonyms/Alias

 CacyBP; Siah-interacting protein 

Gene Name

 Cacybp 

Gene Synonyms/Alias

 Sip 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
16	QKDLEEVKVLLEKST	ubiquitination	[1]
86	YGWDQSDKFVKIYIT	acetylation	[2, 3, 4]
119	RSFDLLVKNLNGKNY	acetylation	[3]
144	ISVESSSKKVKTDTV	ubiquitination	[1]
172	DYLTQVEKECKEKEK	acetylation	[2]
213	DMKRTINKAWVESRE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1) (By similarity). 

Sequence Annotation

 DOMAIN 74 168 CS.
 DOMAIN 169 229 SGS.
 REGION 2 81 Interaction with SIAH1 (By similarity).
 REGION 74 229 Interaction with SKP1 (By similarity).
 REGION 155 229 Interaction with S100A6.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 10 10 N6-acetyllysine (By similarity).
 MOD_RES 21 21 N6-acetyllysine (By similarity).
 MOD_RES 86 86 N6-acetyllysine (By similarity).
 MOD_RES 119 119 N6-acetyllysine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 229 AA 

Protein Sequence

Gene Ontology

 GO:0030877; C:beta-catenin destruction complex; ISS:UniProtKB.
 GO:0044297; C:cell body; IEA:Compara.
 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0005641; C:nuclear envelope lumen; IDA:MGI.
 GO:0007568; P:aging; IEA:Compara.
 GO:0055007; P:cardiac muscle cell differentiation; IEA:Compara.
 GO:0071277; P:cellular response to calcium ion; IEA:Compara.
 GO:0060548; P:negative regulation of cell death; IEA:Compara.
 GO:0045740; P:positive regulation of DNA replication; IEA:Compara.
 GO:0060416; P:response to growth hormone stimulus; IEA:Compara. 

Interpro

 IPR007052; CS-like_domain.
 IPR017447; CS_domain.
 IPR008978; HSP20-like_chaperone.
 IPR007699; SGS.
 IPR015120; Siah-Interact_N. 

Pfam

 PF04969; CS
 PF05002; SGS
 PF09032; Siah-Interact_N 

SMART

  

PROSITE

 PS51203; CS
 PS51048; SGS 

PRINTS