CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004765
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
 AAA52646.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15862.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAC15863.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82319.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; AAF82320.1; CAH71506.1; CAH71506.1; AAH08730.1; AAC25424.1; AAC00172.1; CAA47379.1 
Protein Name
 Hexokinase-1 
Protein Synonyms/Alias
 Brain form hexokinase; Hexokinase type I; HK I 
Gene Name
 HK1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
21LKDDQVKKIDKYLYAubiquitination[1]
187VEGADVVKLLNKAIKubiquitination[1]
191DVVKLLNKAIKKRGDubiquitination[1]
333PELLTRGKFNTSDVSubiquitination[1, 2]
418GVDGSLYKTHPQYSRubiquitination[1]
738EYSLNAGKQRYEKMIubiquitination[2]
763NILIDFTKKGFLFRGubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
 NP_BIND 84 89 ATP 1 (Potential).
 NP_BIND 425 426 ATP 1.
 NP_BIND 532 537 ATP 2.
 NP_BIND 747 748 ATP 2.
 NP_BIND 784 788 ATP 2.
 NP_BIND 863 867 ATP 2.
 REGION 1 12 Hydrophobic.
 REGION 13 475 Regulatory.
 REGION 84 88 Glucose-6-phosphate 1 binding.
 REGION 172 173 Substrate 1 binding.
 REGION 208 209 Substrate 1 binding.
 REGION 291 294 Substrate 1 binding.
 REGION 413 415 Glucose-6-phosphate 1 binding.
 REGION 476 917 Catalytic.
 REGION 532 536 Glucose-6-phosphate 2 binding.
 REGION 603 604 Substrate 2 binding.
 REGION 620 621 Substrate 2 binding.
 REGION 656 657 Substrate 2 binding.
 REGION 682 683 Substrate 2 binding.
 REGION 861 863 Glucose-6-phosphate 2 binding.
 BINDING 30 30 ATP 1.
 BINDING 155 155 Substrate 1.
 BINDING 209 209 Glucose-6-phosphate 1.
 BINDING 232 232 Glucose-6-phosphate 1.
 BINDING 235 235 Substrate 1.
 BINDING 260 260 Substrate 1.
 BINDING 345 345 ATP 1.
 BINDING 449 449 Glucose-6-phosphate 1.
 BINDING 657 657 Glucose-6-phosphate 2.
 BINDING 680 680 ATP 2.
 BINDING 680 680 Glucose-6-phosphate 2.
 BINDING 708 708 Substrate 2.
 BINDING 742 742 Substrate 2.
 BINDING 897 897 Glucose-6-phosphate 2.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 27 27 Phosphotyrosine (By similarity).  
Keyword
 3D-structure; Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Direct protein sequencing; Disease mutation; Glycolysis; Kinase; Membrane; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 917 AA 
Protein Sequence
MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT 60
VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH 120
GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV 180
EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME 240
ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM 300
YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH NAKEILTRLG 360
VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKTH 420
PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA YRLAEQHRQI EETLAHFHLT 480
KDMLLEVKKR MRAEMELGLR KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV 540
LLVKIRSGKK RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF 600
TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD VVAVVNDTVG 660
TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ GQMCINMEWG AFGDNGCLDD 720
IRTHYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISETLKTRG 780
IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA 840
VVDKIRENRG LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG 900
AALITAVGVR LRTEASS 917 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0045121; C:membrane raft; IEA:Compara.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008865; F:fructokinase activity; IBA:RefGenome.
 GO:0004340; F:glucokinase activity; IBA:RefGenome.
 GO:0019158; F:mannokinase activity; IBA:RefGenome.
 GO:0001678; P:cellular glucose homeostasis; IBA:RefGenome.
 GO:0015758; P:glucose transport; TAS:Reactome.
 GO:0006096; P:glycolysis; IBA:RefGenome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome. 
Interpro
 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 
Pfam
 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 
SMART
  
PROSITE
 PS00378; HEXOKINASES 
PRINTS
 PR00475; HEXOKINASE.