CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020350
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 2A 
Protein Synonyms/Alias
 eIF-2A; 65 kDa eukaryotic translation initiation factor 2A 
Gene Name
 EIF2A 
Gene Synonyms/Alias
 CDA02; MSTP004; MSTP089 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
67NIISVTNKGLLHSFDubiquitination[1, 2]
115NLQLYDVKTGTCLKSubiquitination[1, 3]
121VKTGTCLKSFIQKKMubiquitination[1]
127LKSFIQKKMQNWCPSubiquitination[1]
164NFNTIANKLHLQKINubiquitination[1, 4]
169ANKLHLQKINDFVLSubiquitination[1, 2, 4]
183SPGPQPYKVAVYVPGubiquitination[1, 2, 4]
192AVYVPGSKGAPSFVRubiquitination[1, 2, 3, 5]
216PHAALANKSFFKADKubiquitination[2, 4]
305KATIFNLKCDPVFDFubiquitination[1]
347QMEVWDVKNYKLISKubiquitination[4]
350VWDVKNYKLISKPVAubiquitination[1]
354KNYKLISKPVASDSTubiquitination[1]
441EVPNEEPKVATAYRPubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. 
Sequence Annotation
 REPEAT 21 63 WD 1.
 REPEAT 125 163 WD 2.
 REPEAT 356 401 WD 3.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 503 503 Phosphoserine.
 MOD_RES 506 506 Phosphoserine.
 MOD_RES 517 517 Phosphoserine.
 MOD_RES 518 518 Phosphothreonine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Direct protein sequencing; Initiation factor; Phosphoprotein; Polymorphism; Protein biosynthesis; Reference proteome; Repeat; Translation regulation; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 585 AA 
Protein Sequence
MAPSTPLLTV RGSEGLYMVN GPPHFTESTV FPRESGKNCK VCIFSKDGTL FAWGNGEKVN 60
IISVTNKGLL HSFDLLKAVC LEFSPKNTVL ATWQPYTTSK DGTAGIPNLQ LYDVKTGTCL 120
KSFIQKKMQN WCPSWSEDET LCARNVNNEV HFFENNNFNT IANKLHLQKI NDFVLSPGPQ 180
PYKVAVYVPG SKGAPSFVRL YQYPNFAGPH AALANKSFFK ADKVTMLWNK KATAVLVIAS 240
TDVDKTGASY YGEQTLHYIA TNGESAVVQL PKNGPIYDVV WNSSSTEFCA VYGFMPAKAT 300
IFNLKCDPVF DFGTGPRNAA YYSPHGHILV LAGFGNLRGQ MEVWDVKNYK LISKPVASDS 360
TYFAWCPDGE HILTATCAPR LRVNNGYKIW HYTGSILHKY DVPSNAELWQ VSWQPFLDGI 420
FPAKTITYQA VPSEVPNEEP KVATAYRPPA LRNKPITNSK LHEEEPPQNM KPQSGNDKPL 480
SKTALKNQRK HEAKKAAKQE ARSDKSPDLA PTPAPQSTPR NTVSQSISGD PEIDKKIKNL 540
KKKLKAIEQL KEQAATGKQL EKNQLEKIQK ETALLQELED LELGI 585 
Gene Ontology
 GO:0005850; C:eukaryotic translation initiation factor 2 complex; IDA:UniProtKB.
 GO:0043022; F:ribosome binding; IMP:UniProtKB.
 GO:0003743; F:translation initiation factor activity; IMP:UniProtKB.
 GO:0000049; F:tRNA binding; IMP:UniProtKB.
 GO:0009967; P:positive regulation of signal transduction; IEA:Compara.
 GO:0006468; P:protein phosphorylation; IEA:Compara.
 GO:0006417; P:regulation of translation; IMP:UniProtKB.
 GO:0042255; P:ribosome assembly; IMP:UniProtKB.
 GO:0032933; P:SREBP signaling pathway; IEA:Compara. 
Interpro
 IPR011387; TIF2A.
 IPR013979; TIF_beta_prop-like.
 IPR015943; WD40/YVTN_repeat-like_dom. 
Pfam
 PF08662; eIF2A 
SMART
  
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS