UniProt Accession

 EIF3G_HUMAN; O75821; O14801; Q969U5 

Genbank Protein ID

 U96074; AF020833; AF092453; AF094850; BT006889; BC000733; BC008469 

Genbank Nucleotide ID

 AAC78728.1; AAB71866.1; AAG15396.1; AAG15419.1; AAP35535.1; AAH00733.1; AAH08469.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit G 

Protein Synonyms/Alias

 eIF3g; Eukaryotic translation initiation factor 3 RNA-binding subunit; eIF-3 RNA-binding subunit; Eukaryotic translation initiation factor 3 subunit 4; eIF-3-delta; eIF3 p42; eIF3 p44 

Gene Name

 EIF3G 

Gene Synonyms/Alias

 EIF3S4 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
65	EVINGNIKTVTEYKI	ubiquitination	[1, 2]
71	IKTVTEYKIDEDGKK	ubiquitination	[3]
78	KIDEDGKKFKIVRTF	ubiquitination	[3]
148	EEEDPMNKLKGQKIV	ubiquitination	[3]
150	EDPMNKLKGQKIVSC	ubiquitination	[3]
161	IVSCRICKGDHWTTR	acetylation	[4]
161	IVSCRICKGDHWTTR	ubiquitination	[3]
172	WTTRCPYKDTLGPMQ	acetylation	[4]
180	DTLGPMQKELAEQLG	ubiquitination	[1, 2, 5]
193	LGLSTGEKEKLPGEL	acetylation	[4]
193	LGLSTGEKEKLPGEL	ubiquitination	[3]
195	LSTGEKEKLPGELEP	ubiquitination	[3, 5]
209	PVQATQNKTGKYVPP	ubiquitination	[1, 2, 5]
212	ATQNKTGKYVPPSLR	acetylation	[6]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA. 

Sequence Annotation

 DOMAIN 239 317 RRM.
 MOD_RES 38 38 Phosphothreonine.
 MOD_RES 41 41 Phosphothreonine.
 MOD_RES 42 42 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Initiation factor; Nucleus; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 320 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 

Interpro

 IPR017334; eIF3_g.
 IPR024675; eIF3g_N.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF12353; eIF3g
 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS