CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031003
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Centrosomal protein of 95 kDa 
Protein Synonyms/Alias
 cDNA FLJ54025, highly similar to Coiled-coil domain-containing protein 45 
Gene Name
 CEP95 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
44PNEMLSKKALASPSSubiquitination[1]
66YPPSVLSKSRTSFVEubiquitination[1]
150DGLFLISKLPKGSKWubiquitination[1]
156SKLPKGSKWEVYPAQubiquitination[1, 2, 3]
210ELHDVSEKLSQRLSEubiquitination[1, 3, 4]
223SELDWMLKSALGDRIubiquitination[1, 3, 5]
332ERELRRHKVQENIGPubiquitination[1]
360YRGEAVRKGTPECSQubiquitination[1]
424PTLSKMWKQQIAQVEubiquitination[1]
434IAQVEQLKKEACRENubiquitination[1, 3]
446RENRSKKKLQDEIEEubiquitination[1]
466DLLTTLVKKEYEHNKubiquitination[1]
479NKRLQDFKDCIRRQRubiquitination[1]
504QQIVRARKYYDDYRVubiquitination[1]
516YRVQLCAKMMRMRTRubiquitination[1]
530REEMIFKKLFEEGLNubiquitination[1]
540EEGLNIQKQRLRDLRubiquitination[3, 5]
591SQEHQELKAREKSQAubiquitination[3]
653LASFQYSKSPSL***ubiquitination[1, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 657 AA 
Protein Sequence
MLGPSWDGDE AESTGEIIRL GDTAHTFSLR SNGAQCPNEM LSKKALASPS SKSHEDMLYP 60
PSVLSKSRTS FVEDTETLSV SGIPNARKLG EPIRAAIPLH PPYHPSEPRA PCPIGKEYLH 120
SSHCSPAVNS TGEHTEFSGD LDDGLFLISK LPKGSKWEVY PAQVQGPRTR KPPKGKRNEN 180
RATASSCNSP FPQRPRKRLT EQELHDVSEK LSQRLSELDW MLKSALGDRI KEKTDHKEEN 240
TGNEEVEDGT EETLSQHSDG IVEYGPKKSR PGLSMRRKPP YRSHSLSPSP VNKHKQFHLE 300
RKRQRKPRET DVRQFQAQAF TEAFERELRR HKVQENIGPL RIHEKEEETE KIYRGEAVRK 360
GTPECSQPWK IYSRKTTTQS LRGGLPKPNK AVPMKVSEHS LLPLMLEQFP FLYVSGPTLS 420
KMWKQQIAQV EQLKKEACRE NRSKKKLQDE IEEALRRHDL LTTLVKKEYE HNKRLQDFKD 480
CIRRQRLTQS KIKENRQQIV RARKYYDDYR VQLCAKMMRM RTREEMIFKK LFEEGLNIQK 540
QRLRDLRNYA KEKRDEQRRR HQDELDSMEN YYKDQFSLLA EAISQEHQEL KAREKSQAQT 600
LHKVKRELRS KMEKEIQQLQ DMITQNDDDV FFRELEAERF RSRLQLASFQ YSKSPSL 657 
Gene Ontology
 GO:0005813; C:centrosome; IEA:InterPro.
 GO:0000922; C:spindle pole; IEA:InterPro. 
Interpro
 IPR026619; CEP95. 
Pfam
  
SMART
  
PROSITE
  
PRINTS