CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007545
UniProt Accession
Genbank Protein ID
 U06924 
Genbank Nucleotide ID
Protein Name
 Signal transducer and activator of transcription 1 
Protein Synonyms/Alias
  
Gene Name
 Stat1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
138QNTVMLDKQKELDSKubiquitination[1]
193GVAKSDQKQEQLLLHubiquitination[1]
296YEPDPITKNKQVLSDubiquitination[1]
379NTVKGFRKFNILGTHubiquitination[1]
544IPWTRFCKENINDKNubiquitination[1]
592ERERALLKDQQPGTFubiquitination[1]
637AVEPYTKKELSAVTFubiquitination[1]
652PDIIRNYKVMAAENIubiquitination[1]
665NIPENPLKYLYPNIDubiquitination[1]
673YLYPNIDKDHAFGKYubiquitination[1]
679DKDHAFGKYYSRPKEubiquitination[1]
685GKYYSRPKEAPEPMEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN- gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. 
Sequence Annotation
 DOMAIN 573 670 SH2.
 MOD_RES 701 701 Phosphotyrosine; by JAK1, JAK2 or TYK2.
 MOD_RES 727 727 Phosphoserine; by MAPK14 (By similarity).
 CROSSLNK 703 703 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Activator; Complete proteome; Cytoplasm; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; SH2 domain; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 749 AA 
Protein Sequence
MSQWFELQQL DSKFLEQVHQ LYDDSFPMEI RQYLAQWLEK QDWEHAAYDV SFATIRFHDL 60
LSQLDDQYSR FSLENNFLLQ HNIRKSKRNL QDNFQEDPVQ MSMIIYNCLK EERKILENAQ 120
RFNQAQEGNI QNTVMLDKQK ELDSKVRNVK DQVMCIEQEI KTLEELQDEY DFKCKTSQNR 180
EGEANGVAKS DQKQEQLLLH KMFLMLDNKR KEIIHKIREL LNSIELTQNT LINDELVEWK 240
RRQQSACIGG PPNACLDQLQ TWFTIVAETL QQIRQQLKKL EELEQKFTYE PDPITKNKQV 300
LSDRTFLLFQ QLIQSSFVVE RQPCMPTHPQ RPLVLKTGVQ FTVKSRLLVK LQESNLLTKV 360
KCHFDKDVNE KNTVKGFRKF NILGTHTKVM NMEESTNGSL AAELRHLQLK EQKNAGNRTN 420
EGPLIVTEEL HSLSFETQLC QPGLVIDLET TSLPVVVISN VSQLPSGWAS ILWYNMLVTE 480
PRNLSFFLNP PCAWWSQLSE VLSWQFSSVT KRGLNADQLS MLGEKLLGPN AGPDGLIPWT 540
RFCKENINDK NFSFWPWIDT ILELIKNDLL CLWNDGCIMG FISKERERAL LKDQQPGTFL 600
LRFSESSREG AITFTWVERS QNGGEPDFHA VEPYTKKELS AVTFPDIIRN YKVMAAENIP 660
ENPLKYLYPN IDKDHAFGKY YSRPKEAPEP MELDDPKRTG YIKTELISVS EVHPSRLQTT 720
DNLLPMSPEE FDEMSRIVGP EFDSMMSTV 749 
Gene Ontology
 GO:0030424; C:axon; ISS:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030425; C:dendrite; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IMP:MGI.
 GO:0004871; F:signal transducer activity; IEA:InterPro.
 GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; IMP:UniProtKB.
 GO:0008015; P:blood circulation; ISS:UniProtKB.
 GO:0019221; P:cytokine-mediated signaling pathway; IDA:MGI.
 GO:0006917; P:induction of apoptosis; ISS:UniProtKB.
 GO:0031663; P:lipopolysaccharide-mediated signaling pathway; IDA:MGI.
 GO:0072162; P:metanephric mesenchymal cell differentiation; ISS:UniProtKB.
 GO:0072136; P:metanephric mesenchymal cell proliferation involved in metanephros development; ISS:UniProtKB.
 GO:0003340; P:negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis; ISS:UniProtKB.
 GO:0072308; P:negative regulation of metanephric nephron tubule epithelial cell differentiation; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0002053; P:positive regulation of mesenchymal cell proliferation; ISS:UniProtKB.
 GO:0048661; P:positive regulation of smooth muscle cell proliferation; ISS:UniProtKB.
 GO:0061326; P:renal tubule development; ISS:UniProtKB.
 GO:0051591; P:response to cAMP; ISS:UniProtKB.
 GO:0043330; P:response to exogenous dsRNA; IDA:MGI.
 GO:0043434; P:response to peptide hormone stimulus; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IMP:MGI. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR008967; p53-like_TF_DNA-bd.
 IPR000980; SH2.
 IPR022752; STAT1_TAZ2-bd_C.
 IPR013800; STAT_TF_alpha.
 IPR015988; STAT_TF_coiled-coil.
 IPR001217; STAT_TF_core.
 IPR013801; STAT_TF_DNA-bd.
 IPR012345; STAT_TF_DNA-bd_sub.
 IPR013799; STAT_TF_prot_interaction. 
Pfam
 PF00017; SH2
 PF12162; STAT1_TAZ2bind
 PF01017; STAT_alpha
 PF02864; STAT_bind
 PF02865; STAT_int 
SMART
 SM00252; SH2
 SM00964; STAT_int 
PROSITE
 PS50001; SH2 
PRINTS