CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013508
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 C-Jun-amino-terminal kinase-interacting protein 4 
Protein Synonyms/Alias
 JIP-4; JNK-interacting protein 4; JNK-associated leucine-zipper protein; JLP; JNK/SAPK-associated protein 2; JSAP2; Mitogen-activated protein kinase 8-interacting protein 4; Sperm-associated antigen 9 
Gene Name
 Spag9 
Gene Synonyms/Alias
 Jip4; Jsap2; Kiaa0516; Mapk8ip4 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
198NLPRILRKERPISLGubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 109 109 Phosphoserine (By similarity).
 MOD_RES 183 183 Phosphoserine (By similarity).
 MOD_RES 185 185 Phosphoserine (By similarity).
 MOD_RES 194 194 Phosphoserine (By similarity).
 MOD_RES 203 203 Phosphoserine.
 MOD_RES 217 217 Phosphothreonine.
 MOD_RES 244 244 Phosphothreonine.
 MOD_RES 251 251 Phosphoserine (By similarity).
 MOD_RES 265 265 Phosphoserine (By similarity).
 MOD_RES 268 268 Phosphoserine (By similarity).
 MOD_RES 272 272 Phosphoserine (By similarity).
 MOD_RES 292 292 Phosphothreonine.
 MOD_RES 311 311 Phosphoserine (By similarity).
 MOD_RES 329 329 Phosphoserine (By similarity).
 MOD_RES 332 332 Phosphoserine (By similarity).
 MOD_RES 347 347 Phosphoserine (By similarity).
 MOD_RES 348 348 Phosphothreonine (By similarity).
 MOD_RES 365 365 Phosphothreonine.
 MOD_RES 418 418 Phosphothreonine (By similarity).
 MOD_RES 586 586 Phosphothreonine (By similarity).
 MOD_RES 730 730 Phosphoserine.
 MOD_RES 733 733 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1321 AA 
Protein Sequence
MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 60
LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 120
VESLESQTRQ LELKAKNYAD QISMNPGCML LFVFGFVGGA VVINSAILVS LSVLLLVHFS 180
ISTGVPALTQ NLPRILRKER PISLGIFPLP AGDGLLTPDT QKGGETPGSE QWKFQELSQP 240
RSHTSLKDEL SDISQGGSKA TTPASTANSD VSAIPPDTPS KEDNEGFVKG TDTSNKSEIS 300
KHIEVQVAQE TRNVSTESGE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA 360
FDRNTESLFE ELSSAGSGLI GDVDEGADLL GMGREVENLI LENTQLLETK NALNVVKNDL 420
IAKVDELTCE KDVLQGELEA VKQAKLKLED KNRELEEELR KARAEAEDAR QKAKDDDDSD 480
IPTAQRKRFT RVEMARVLME RNQYKERLME LQEAVRWTEM IRASRENPAM QEKKRSSIWQ 540
FFSRLFSSSS NATKKPEPPV NLKYNAPTSH VTPSVKKRSS TLSQLPGDKS KAFDFLSEET 600
EASLASRREQ KREQYRQVKA HVQKEDGRVQ AFGWSLPQKY KQVANGQGET KMKNLPVPVY 660
LRPLDEKDAS MKLWCAVGVN LSGGKTRDGG SVVGASVFYK DIAGLDTEGS KQRSASQSSL 720
DKLDQELKEQ QKEFKNQEEL SSQVWICTST HSTTKVIIID AVQPGNILDS FTVCNSHVLC 780
IASVPGARET DYPAGEELSE SGQVDKASLC GSMTSNSSAE MDSLLGGITV VGCSTEGLTG 840
AATSPSTNGA SPVIEKPPEM ETENSEVDEN IPTAEEATEA TEGNAGSTED TVDISQPGVY 900
TEHVFTDPLG VQIPEDLSPV FQSSNDSDVY KDQISVLPNE QDLAREEAQK MSSLLPTMWL 960
GAQNGCLYVH SSVAQWRKCL HSIKLKDSIL SIVHVKGIVL VALADGTLAI FHRGVDGQWD 1020
LSNYHLLDLG RPHHSIRCMT VVHDKVWCGY RNKIYVVQPK AMKIEKSFDA HPRKESQVRQ 1080
LAWVGDGVWV SIRLDSTLRL YHAHTYQHLQ DVDIEPYVSK MLGTGKLGFS FVRITALMVS 1140
CNRLWVGTGN GVIISIPLTE TNKTSGTPGN RPGSVIRVYG DENSDKVTPG TFIPYCSMAH 1200
AQLCFHGHRD AVKFFVAVPG QVISPQSSSG GADLTADKAG SSAQEPSSQT PLKSMLVISG 1260
GEGYIDFRMG DEGGESELLG EDLPLEPSVT KAERSHLIVW QVMCGNE 1307 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
 GO:0008432; F:JUN kinase binding; IDA:MGI.
 GO:0019894; F:kinesin binding; IDA:MGI.
 GO:0048273; F:mitogen-activated protein kinase p38 binding; IDA:MGI.
 GO:0007257; P:activation of JUN kinase activity; IDA:MGI.
 GO:0000187; P:activation of MAPK activity; IDA:MGI.
 GO:0090074; P:negative regulation of protein homodimerization activity; IDA:MGI.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0045666; P:positive regulation of neuron differentiation; IMP:MGI.
 GO:0051260; P:protein homooligomerization; IDA:MGI.
 GO:0042147; P:retrograde transport, endosome to Golgi; IEA:Compara.
 GO:0051146; P:striated muscle cell differentiation; IGI:MGI. 
Interpro
 IPR019143; JNK/Rab-associated_protein-1_N.
 IPR011044; Quino_amine_DH_bsu.
 IPR015943; WD40/YVTN_repeat-like_dom. 
Pfam
 PF09744; Jnk-SapK_ap_N 
SMART
  
PROSITE
  
PRINTS