UniProt Accession

 NACB1_YEAST; Q02642; D6W3X7; Q02107 

Genbank Protein ID

 L05185; S49596; X78725; U44030; AY558440; J04421; BK006949 

Genbank Nucleotide ID

 AAB63973.1; AAB24290.1; CAA55371.1; AAB68183.1; AAS56766.1; DAA11393.1 

Protein Name

 Nascent polypeptide-associated complex subunit beta-1 

Protein Synonyms/Alias

 NAC-beta-1; BTF3 homolog EGD1; Beta-1-NAC; GAL4 DNA-binding enhancer protein 1 

Gene Name

 EGD1 

Gene Synonyms/Alias

 YPL037C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
43	GANKDDTKLQSQLAK	acetylation	[1]
70	NFFKDDGKVMHFNKV	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Component of the nascent polypeptide-associated complex (NAC), a dynamic component of the ribosomal exit tunnel, protecting the emerging polypeptides from interaction with other cytoplasmic proteins to ensure appropriate nascent protein targeting. The NAC complex also promotes mitochondrial protein import by enhancing productive ribosome interactions with the outer mitochondrial membrane and blocks the inappropriate interaction of ribosomes translating non-secretory nascent polypeptides with translocation sites in the membrane of the endoplasmic reticulum EGD1 may act as a transcription factor that exert a negative effect on the expression of several genes that are transcribed by RNA polymerase II. Can enhance DNA binding of the GAL4 protein activator. 

Sequence Annotation

 DOMAIN 38 103 NAC-A/B.
 MOD_RES 151 151 Phosphothreonine.  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repressor; Transcription; Transcription regulation; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 157 AA 

Protein Sequence

Gene Ontology

 GO:0005854; C:nascent polypeptide-associated complex; IDA:SGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0051082; F:unfolded protein binding; IMP:SGD.
 GO:0051083; P:'de novo' cotranslational protein folding; IPI:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR027129; Btt1_fungal.
 IPR002715; Nas_poly-pep-assoc_cplx_dom. 

Pfam

 PF01849; NAC 

SMART

  

PROSITE

 PS51151; NAC_AB 

PRINTS