UniProt Accession

 MTHFS_HUMAN; P49914; H3BQ75 

Genbank Protein ID

 L38928; AC015871; AC021483; BC019921; CK002935 

Genbank Nucleotide ID

 AAC41945.1; AAH19921.1 

Protein Name

 5-formyltetrahydrofolate cyclo-ligase 

Protein Synonyms/Alias

 5,10-methenyl-tetrahydrofolate synthetase; MTHFS; Methenyl-THF synthetase 

Gene Name

 MTHFS 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
10	AAAVSSAKRSLRGEL	ubiquitination	[1, 2]
39	QSRVLSQKVIAHSEY	ubiquitination	[2]
76	KDIFQRGKICFIPRY	ubiquitination	[2]
150	GNRLGRGKGYYDAYL	ubiquitination	[2]
158	GYYDAYLKRCLQHQE	ubiquitination	[2]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. 

Sequence Annotation

 NP_BIND 10 14 ATP.
 NP_BIND 147 154 ATP.
 REGION 148 152 Substrate binding.
 BINDING 61 61 Substrate.
 BINDING 109 109 Substrate.
 BINDING 189 189 ATP.
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Folate-binding; Ligase; Magnesium; Nucleotide-binding; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 203 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; NAS:UniProtKB.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0030272; F:5-formyltetrahydrofolate cyclo-ligase activity; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005542; F:folic acid binding; IEA:UniProtKB-KW.
 GO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro.
 GO:0015942; P:formate metabolic process; NAS:UniProtKB.
 GO:0046653; P:tetrahydrofolate metabolic process; IDA:UniProtKB. 

Interpro

 IPR002698; FTHF_cligase.
 IPR024185; FTHF_cligase-like. 

Pfam

 PF01812; 5-FTHF_cyc-lig 

SMART

  

PROSITE

  

PRINTS