CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005639
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 H/ACA ribonucleoprotein complex subunit 1 
Protein Synonyms/Alias
 snoRNP protein GAR1 
Gene Name
 GAR1 
Gene Synonyms/Alias
 YHR089C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
59VCRSINTKIPYFNAPubiquitination[1]
77ENKTQVGKVDEILGPubiquitination[2]
115KFYIAADKLLPIERFacetylation[3]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs. Essential for growth. 
Sequence Annotation
 REGION 4 21 RGG-box 1.
 REGION 147 205 RGG-box 2.
 MOD_RES 4 4 Asymmetric dimethylarginine (Probable).
 MOD_RES 8 8 Asymmetric dimethylarginine (Probable).
 MOD_RES 11 11 Asymmetric dimethylarginine (Probable).
 MOD_RES 15 15 Asymmetric dimethylarginine (Probable).
 MOD_RES 19 19 Asymmetric dimethylarginine (Probable).
 MOD_RES 147 147 Asymmetric dimethylarginine (Probable).
 MOD_RES 154 154 Asymmetric dimethylarginine (Probable).
 MOD_RES 158 158 Asymmetric dimethylarginine (Probable).
 MOD_RES 162 162 Asymmetric dimethylarginine (Probable).
 MOD_RES 165 165 Asymmetric dimethylarginine (Probable).
 MOD_RES 171 171 Asymmetric dimethylarginine (Probable).
 MOD_RES 174 174 Asymmetric dimethylarginine (Probable).
 MOD_RES 180 180 Asymmetric dimethylarginine (Probable).
 MOD_RES 184 184 Asymmetric dimethylarginine (Probable).
 MOD_RES 189 189 Asymmetric dimethylarginine (Probable).
 MOD_RES 193 193 Asymmetric dimethylarginine (Probable).
 MOD_RES 197 197 Asymmetric dimethylarginine (Probable).
 MOD_RES 201 201 Asymmetric dimethylarginine (Probable).  
Keyword
 3D-structure; Complete proteome; Methylation; Nucleus; Reference proteome; Repeat; Ribonucleoprotein; Ribosome biogenesis; RNA-binding; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 205 AA 
Protein Sequence
MSFRGGNRGG RGGFRGGFRG GRTGSARSFQ QGPPDTVLEM GAFLHPCEGD IVCRSINTKI 60
PYFNAPIYLE NKTQVGKVDE ILGPLNEVFF TIKCGDGVQA TSFKEGDKFY IAADKLLPIE 120
RFLPKPKVVG PPKPKNKKKR SGAPGGRGGA SMGRGGSRGG FRGGRGGSSF RGGRGGSSFR 180
GGSRGGSFRG GSRGGSRGGF RGGRR 205 
Gene Ontology
 GO:0031429; C:box H/ACA snoRNP complex; IPI:UniProtKB.
 GO:0034513; F:box H/ACA snoRNA binding; IPI:SGD.
 GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
 GO:0006364; P:rRNA processing; TAS:SGD.
 GO:0031120; P:snRNA pseudouridine synthesis; IDA:SGD. 
Interpro
 IPR007504; H/ACA_rnp_Gar1/Naf1.
 IPR021154; H/ACA_rnp_Gar1_euk.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF04410; Gar1 
SMART
  
PROSITE
  
PRINTS